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Olfactomedin 1 ELISA Kit

OLFM1 Reaktivität: Maus Colorimetric Sandwich ELISA 0.084 ng/mL - 20 ng/mL Cell Culture Supernatant, Plasma, Serum
Produktnummer ABIN6730244
  • Target Alle Olfactomedin 1 (OLFM1) ELISA Kits anzeigen
    Olfactomedin 1 (OLFM1)
    Reaktivität
    Maus
    Nachweismethode
    Colorimetric
    Methodentyp
    Sandwich ELISA
    Detektionsbereich
    0.084 ng/mL - 20 ng/mL
    Untere Nachweisgrenze
    0.084 ng/mL
    Applikation
    ELISA
    Verwendungszweck
    Mouse Olfactomedin-1 ELISA Kit for Serum, Plasma, and Cell Culture Supernatants.
    Proben
    Cell Culture Supernatant, Plasma, Serum
    Analytische Methode
    Quantitative
    Spezifität
    This ELISA antibody pair recognizes Mouse Olfactomedin-1.
    Produktmerkmale
    • Strip plates and additional reagents allow for use in multiple experiments
    • Quantitative protein detection
    • Establishes normal range
    • The best products for confirmation of antibody array data
    Bestandteile
    • Pre-Coated 96-well Strip Microplate
    • Wash Buffer
    • Stop Solution
    • Assay Diluent(s)
    • Lyophilized Standard
    • Biotinylated Detection Antibody
    • Streptavidin-Conjugated HRP
    • TMB One-Step Substrate
    Benötigtes Material
    • Distilled or deionized water
    • Precision pipettes to deliver 2 µl to 1 µl volumes
    • Adjustable 1-25 µl pipettes for reagent preparation
    • 100 µl and 1 liter graduated cylinders
    • Tubes to prepare standard and sample dilutions
    • Absorbent paper
    • Microplate reader capable of measuring absorbance at 450nm
    • Log-log graph paper or computer and software for ELISA data analysis
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  • Applikationshinweise
    Optimal working dilution should be determined by the investigator.
    Protokoll
    1. Prepare all reagents, samples and standards as instructed in the manual.
    2. Add 100 µl of standard or sample to each well.
    3. Incubate 2.5 h at RT or O/N at 4°C.
    4. Add 100 µl of prepared biotin antibody to each well.
    5. Incubate 1 h at RT.
    6. Add 100 µl of prepared Streptavidin solution to each well.
    7. Incubate 45 min at RT.
    8. Add 100 µl of TMB One-Step Substrate Reagent to each well.
    9. Incubate 30 min at RT.
    10. Add 50 µl of Stop Solution to each well.
    11. Read at 450 nm immediately.
    Aufbereitung der Reagenzien

    Recommended Dilution for serum and plasma samples10 fold

    Beschränkungen
    Nur für Forschungszwecke einsetzbar
  • Informationen zur Lagerung
    The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
    Haltbarkeit
    6 months
  • Target Alle Olfactomedin 1 (OLFM1) ELISA Kits anzeigen
    Olfactomedin 1 (OLFM1)
    Andere Bezeichnung
    Olfactomedin-1 (OLFM1 Produkte)
    Synonyme
    MGC132187 ELISA Kit, OLFM1 ELISA Kit, amy ELISA Kit, noe1 ELISA Kit, olfa ELISA Kit, noelin1 ELISA Kit, noelin-1 ELISA Kit, AMY ELISA Kit, NOE1 ELISA Kit, NOELIN1 ELISA Kit, OlfA ELISA Kit, AW742568 ELISA Kit, Noe1 ELISA Kit, Pancortin ELISA Kit, Pancortin3 ELISA Kit, D2Sut1e ELISA Kit, NOELIN-1 ELISA Kit, olfactomedin 1 ELISA Kit, olfactomedin 1 S homeolog ELISA Kit, OLFM1 ELISA Kit, olfm1.S ELISA Kit, olfm1 ELISA Kit, Olfm1 ELISA Kit
    UniProt
    O88998
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