ACPP ELISA Kit

Produktnummer ABIN6730056

Produktdetails ACPP ELISA Kit

Target: ACPP (Acid Phosphatase, Prostate (ACPP))

Reaktivität: Maus

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Applikation: ELISA

Verwendungszweck: Mouse ACPP ELISA Kit.

Proben: Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate

Analytische Methode: Quantitative

Spezifität: This ELISA antibody pair recognizes Mouse ACPP.

Produktmerkmale:

• Strip plates and additional reagents allow for use in multiple experiments
• Quantitative protein detection
• Establishes normal range
• The best products for confirmation of antibody array data

Bestandteile:

• Pre-Coated 96-well Strip Microplate
• Wash Buffer
• Stop Solution
• Assay Diluent(s)
• Lyophilized Standard
• Biotinylated Detection Antibody
• Streptavidin-Conjugated HRP
• TMB One-Step Substrate

Benötigtes Material:

• Distilled or deionized water
• Precision pipettes to deliver 2 µl to 1 µl volumes
• Adjustable 1-25 µl pipettes for reagent preparation
• 100 µl and 1 liter graduated cylinders
• Tubes to prepare standard and sample dilutions
• Absorbent paper
• Microplate reader capable of measuring absorbance at 450nm
• Log-log graph paper or computer and software for ELISA data analysis

Anwendungsinformationen

Applikationshinweise: Optimal working dilution should be determined by the investigator.

Protokoll:

1. Prepare all reagents, samples and standards as instructed in the manual.
2. Add 100 µl of standard or sample to each well.
3. Incubate 2.5 h at RT or O/N at 4°C.
4. Add 100 µl of prepared biotin antibody to each well.
5. Incubate 1 h at RT.
6. Add 100 µl of prepared Streptavidin solution to each well.
7. Incubate 45 min at RT.
8. Add 100 µl of TMB One-Step Substrate Reagent to each well.
9. Incubate 30 min at RT.
10. Add 50 µl of Stop Solution to each well.
11. Read at 450 nm immediately.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Informationen zur Lagerung: The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.

Haltbarkeit: 6 months

Details zu ACPP

Target: ACPP (Acid Phosphatase, Prostate (ACPP))

Andere Bezeichnung: ACPP (ACPP Produkte)

Synonyme: 5'-NT ELISA Kit, ACP-3 ELISA Kit, ACP3 ELISA Kit, PAP ELISA Kit, AMAP2 ELISA Kit, CENTB3 ELISA Kit, DDEF2 ELISA Kit, PAG3 ELISA Kit, Pap-alpha ELISA Kit, SHAG1 ELISA Kit, A030005E02Rik ELISA Kit, FRAP ELISA Kit, Lap ELISA Kit, Ppal ELISA Kit, Acpp11 ELISA Kit, RNACPP11 ELISA Kit, pap ELISA Kit, ACPP ELISA Kit, acp-3 ELISA Kit, acp3 ELISA Kit, acpt ELISA Kit, acid phosphatase, prostate ELISA Kit, ArfGAP with SH3 domain, ankyrin repeat and PH domain 2 ELISA Kit, prostatic acid phosphatase ELISA Kit, prostatic acid phosphatase, putative ELISA Kit, acid phosphatase, prostate S homeolog ELISA Kit, ACPP ELISA Kit, ASAP2 ELISA Kit, Acpp ELISA Kit, CpipJ_CPIJ004002 ELISA Kit, Smp_016640 ELISA Kit, acpp.S ELISA Kit

UniProt: Q8CE08

Pathways: Synaptic Membrane, Ribonucleoside Biosynthetic Process