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Tryptase-5 ELISA Kit

This anti-Tryptase-5 antibody (ABIN6730052) is a antibody detecting Tryptase-5 in ELISA. Suitable for Mouse.
Produktnummer ABIN6730052

Kurzübersicht für Tryptase-5 ELISA Kit (ABIN6730052)

Target

Tryptase-5 (PRSS32) (Protease, Serine, 32 (PRSS32))

Reaktivität

Maus

Nachweismethode

Colorimetric

Methodentyp

Sandwich ELISA

Applikation

ELISA

Proben

Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate
  • Verwendungszweck

    Mouse Tryptase-5 ELISA Kit.

    Analytische Methode

    Quantitative

    Spezifität

    This ELISA antibody pair recognizes Mouse Tryptase-5.

    Produktmerkmale

    • Strip plates and additional reagents allow for use in multiple experiments
    • Quantitative protein detection
    • Establishes normal range
    • The best products for confirmation of antibody array data

    Bestandteile

    • Pre-Coated 96-well Strip Microplate
    • Wash Buffer
    • Stop Solution
    • Assay Diluent(s)
    • Lyophilized Standard
    • Biotinylated Detection Antibody
    • Streptavidin-Conjugated HRP
    • TMB One-Step Substrate

    Benötigtes Material

    • Distilled or deionized water
    • Precision pipettes to deliver 2 µl to 1 µl volumes
    • Adjustable 1-25 µl pipettes for reagent preparation
    • 100 µl and 1 liter graduated cylinders
    • Tubes to prepare standard and sample dilutions
    • Absorbent paper
    • Microplate reader capable of measuring absorbance at 450nm
    • Log-log graph paper or computer and software for ELISA data analysis
  • Applikationshinweise

    Optimal working dilution should be determined by the investigator.

    Protokoll

    1. Prepare all reagents, samples and standards as instructed in the manual.
    2. Add 100 µl of standard or sample to each well.
    3. Incubate 2.5 h at RT or O/N at 4°C.
    4. Add 100 µl of prepared biotin antibody to each well.
    5. Incubate 1 h at RT.
    6. Add 100 µl of prepared Streptavidin solution to each well.
    7. Incubate 45 min at RT.
    8. Add 100 µl of TMB One-Step Substrate Reagent to each well.
    9. Incubate 30 min at RT.
    10. Add 50 µl of Stop Solution to each well.
    11. Read at 450 nm immediately.

    Beschränkungen

    Nur für Forschungszwecke einsetzbar
  • Informationen zur Lagerung

    The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.

    Haltbarkeit

    6 months
  • Target

    Tryptase-5 (PRSS32) (Protease, Serine, 32 (PRSS32))

    Andere Bezeichnung

    Tryptase-5

    UniProt

    E9Q409
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