FURIN ELISA Kit

Produktnummer ABIN6720393

Hersteller Produkt- Nr.: koa0553

Das Human FURIN ELISA Kit (ABIN6720393) ist ein Colorimetric ELISA Kit zur Detektion von Human FURIN.

Kurzübersicht für FURIN ELISA Kit (ABIN6720393)

Target: FURIN (Furin (Paired Basic Amino Acid Cleaving Enzyme) (FURIN))

Reaktivität: Human

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Detektionsbereich: 312 pg/mL - 20000 pg/mL

Applikation: ELISA

Proben: Cell Culture Supernatant, Cell Lysate

Produktdetails FURIN ELISA Kit

Untere Nachweisgrenze: 312 pg/mL

Hersteller Produkt- Nr.: koa0553

Hersteller: Rockland

Verwendungszweck: Human Furin Sandwich ELISA Kit for Quantitative Detection

Marke: AccuSignal™

Analytische Methode: Quantitative

Spezifität: Expression system for standard: NS0
Immunogen sequence: D108-E715

Kreuzreaktivität (Details): There is no detectable cross-reactivity with other relevant proteins.

Sensitivität: < 10 pg/mL

Bestandteile:

• Antibody-coated 96-well plate
• Target Protein Standard
• Detection antibody
• Detection reagent
• Diluent buffers
• Wash buffers
• Substrate Solution
• Stop solutions
• Adhesive covers

Benötigtes Material: Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional). Pipettes and pipette tips capable of precisely dispensing 0.5 μL through 1 mL volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500 mL graduated cylinders. Test tubes for dilution.

Anwendungsinformationen

Kommentare:

Capture antibody and Detection antibody: monoclonal antibody from mouse, polyclonal antibody from goat

Probenmenge: 100 μL

Plattentyp: Pre-coated

Protokoll: Useful in Sandwich ELISA for Quantitative Detection of Antigen. Aliquot 0.1 mL per well of the 20000pg/mL, 10000pg/mL, 5000pg/mL, 2500pg/mL, 1250pg/mL, 625pg/mL, 312pg/mL human Furin standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of human cell culture supernates, tissue homogenates or cell lysates to each empty well. It is recommended that each human Furin standard solution and each sample be measured in duplicate.

Testpräzision: Intra-Assay Precision (CV%): 5.2%, 5.2%, 5.6%
Inter-Assay Precision (CV%): 5.5%, 5.5%, 5.7%

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store vials at 4°C prior to opening. Centrifuge product if not completely clear after standing at room temperature. This product is stable for 6 months at 4°C as an undiluted liquid. Dilute only prior to immediate use. For extended storage freeze at -20°C or below for 12 months. Avoid cycles of freezing and thawing.

Haltbarkeit: 12 months

Details zu FURIN

Target: FURIN (Furin (Paired Basic Amino Acid Cleaving Enzyme) (FURIN))

Andere Bezeichnung: Furin

Hintergrund:

Background: Furin (Fur), also known as PACE, is a protein that in humans is encoded by the FURIN gene. The protein encoded by this gene is an enzyme which belongs to the subtilisin-like proprotein convertase family. The FUR gene is located approximately 1 kb upstream of the FES gene on chromosome 15q25-q26. This gene is thought to play a role in tumor progression. Furin is one of the proteases responsible for the proteolytic cleavage of HIV envelope polyprotein precursor gp160 to gp120 and gp41 prior to viral assembly. Furin is enriched in the Golgi apparatus, where it functions to cleave other proteins into their mature/active forms. Expression of furin in T-cells is required for maintenance of peripheral immune tolerance

Gene Full Name: furin, paired basic amino acid cleaving enzyme

Gen-ID: 5045

NCBI Accession: NP_001276752

UniProt: P09958

Pathways: Notch Signalweg, Neurotrophin Signalübertragung