MMP7 ELISA Kit

Produktnummer ABIN6720386

Hersteller Produkt- Nr.: koa0271

Dieses Colorimetric ELISA-Kit wurde entwickelt für die quantitative Messung von Human MMP7.

Kurzübersicht für MMP7 ELISA Kit (ABIN6720386)

Target: MMP7 (Matrix Metallopeptidase 7 (Matrilysin, Uterine) (MMP7))

Reaktivität: Human

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Detektionsbereich: 156 pg/mL - 10000 pg/mL

Applikation: ELISA

Proben: Cell Culture Supernatant, Plasma (heparin), Saliva, Serum, Urine

Produktdetails MMP7 ELISA Kit

Untere Nachweisgrenze: 156 pg/mL

Hersteller Produkt- Nr.: koa0271

Hersteller: Rockland

Verwendungszweck: Human MMP-7 Sandwich ELISA Kit for Quantitative Detection

Marke: AccuSignal™

Analytische Methode: Quantitative

Spezifität:

Production: Natural and recombinant human total MMP-7. There is no detectable cross-reactivity with other relevant proteins.

Sensitivität: < 6 pg/mL

Bestandteile:

• Antibody-coated 96-well plate
• Target Protein Standard
• Detection antibody
• Detection reagent
• Diluent buffers
• Wash buffers
• Substrate Solution
• Stop solutions
• Adhesive covers

Anwendungsinformationen

Applikationshinweise: Useful in Sandwich ELISA for Quantitative Detection of Antigen. Aliquot 0.1 mL per well of the 10000pg/mL, 5000pg/mL, 2500pg/mL, 1250pg/mL, 625pg/mL, 312pg/mL, 156pg/mL human MMP-7 standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of human cell culture supernates, serum, plasma(heparin), saliva or urine to each empty well. It is recommended that each human MMP-7 standard solution and each sample be measured in duplicate.

Kommentare:

Standard: Expression system for standard: E.coli, Immunogen sequence:  L18-K267

Probenmenge: 100 μL

Plattentyp: Pre-coated

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store vials at 4°C prior to opening. Centrifuge product if not completely clear after standing at room temperature. This product is stable for 6 months at 4°C as an undiluted liquid. Dilute only prior to immediate use. For extended storage freeze at -20°C or below for 12 months. Avoid cycles of freezing and thawing.

Haltbarkeit: 12 months

Details zu MMP7

Target: MMP7 (Matrix Metallopeptidase 7 (Matrilysin, Uterine) (MMP7))

Andere Bezeichnung: MMP-7

Hintergrund:

Synonyms: Matrilysin, Matrin, Matrix metalloproteinase 7, Matrix metalloproteinase-7, MMP-7, MMP7, MMP7_HUMAN, MPSL1, Pump 1 protease, Pump-1 protease, PUMP1, Uterine metalloproteinase

Background: Matrix metalloproteinase-7(MMP-7) previously called putative metalloproteinase I(PUMP1) or matrilysin. The PUMP1 gene has been identified through studies of collagenase-related connective-tissue-degrading metalloproteinases produced by human tumors. The PUMP I protein has 267 amino acids and is significantly shorter than stromelysin or collagenase(477 and 469 amino acids, respectively). Matrix metalloproteinases play a crucial role in tumor invasion and metastasis. Matrilysin, a member of the matrix metalloproteinase family, is structurally different from the other matrix metalloproteinases by virtue of the absence of a conserved COOH-terminal protein domain. In addition, matrilysin mRNA is regulated in a specific and distinct manner in normal and malignant tissues. Matrilysin has been shown to correlate with nodal or distant metastasis in colorectal carcinomas, however, its implication in early invasive colorectal carcinomas has not been determined.1 Matrilysin is also a mediator of pulmonary fibrosis and a ...

Gen-ID: 4316

NCBI Accession: NP_002414

UniProt: P09237

Pathways: Production of Molecular Mediator of Immune Response