CD163 ELISA Kit

Produktnummer ABIN6720374

Hersteller Produkt- Nr.: koa0658

Dieses Colorimetric ELISA-Kit wurde entwickelt für die quantitative Messung von Maus CD163.

Kurzübersicht für CD163 ELISA Kit (ABIN6720374)

Target: CD163

Reaktivität: Maus

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Detektionsbereich: 0.78 ng/mL - 50 ng/mL

Applikation: ELISA

Proben: Cell Culture Supernatant, Plasma (heparin), Serum

Produktdetails CD163 ELISA Kit

Untere Nachweisgrenze: 0.78 ng/mL

Hersteller Produkt- Nr.: koa0658

Hersteller: Rockland

Verwendungszweck: Mouse CD163 Sandwich ELISA Kit for Quantitative Detection

Marke: AccuSignal™

Analytische Methode: Quantitative

Spezifität:

Production: Natural and recombinant mouse CD163. There is no detectable cross-reactivity with other relevant proteins.

Sensitivität: < 20 pg/mL

Bestandteile:

• Antibody-coated 96-well plate
• Target Protein Standard
• Detection antibody
• Detection reagent
• Diluent buffers
• Wash buffers
• Substrate Solution
• Stop solutions
• Adhesive covers

Anwendungsinformationen

Applikationshinweise: Useful in Sandwich ELISA for Quantitative Detection of Antigen. Aliquot 0.1 mL per well of the 50 ng/mL, 25 ng/mL, 12.5 ng/mL, 6.25 ng/mL, 3.12 ng/mL, 1.56 ng/mL, 0.78 ng/mL mouse CD163 standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of mouse cell culture supernates, serum, plasma(heparin) to each empty well. We recommend that each mouse CD163 standard solution and each sample is measured in duplicate.

Kommentare:

Standard: Expression system for standard: NSO, Immunogen sequence: V39-T1045

Probenmenge: 100 μL

Plattentyp: Pre-coated

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store vials at 4°C prior to opening. Centrifuge product if not completely clear after standing at room temperature. This product is stable for 6 months at 4°C as an undiluted liquid. Dilute only prior to immediate use. For extended storage freeze at -20°C or below for 12 months. Avoid cycles of freezing and thawing.

Haltbarkeit: 12 months

Details zu CD163

Target: CD163

Andere Bezeichnung: CD163

Hintergrund:

Synonyms: CD_antigen=CD163, CD163, Hemoglobin scavenger receptor, M130, MM130, Scavenger receptor cysteine-rich type 1 protein M130

Background: CD163(Cluster of Differentiation 163) is a human protein encoded by the CD163 gene. It has also been shown to mark cells of monocyte/macrophage lineage. CD163, a member of the scavenger receptor cysteine-rich(SRCR) superfamily, is exclusively expressed by monocytes and macrophages. Using FISH, somatic cell hybrid analysis, and radiation hybrid analysis, CD163 gene was mapped the to chromosome 12p13.3. CD163 is upregulated in a large range of diseases inflammatory diseases including type 2 diabetes, macrophage activation sickness, Tangier's disease, rheumatoid arthritis etc.

Gen-ID: 93671

NCBI Accession: NP_444324

UniProt: Q2VLH6