PRSS8 ELISA Kit

Produktnummer ABIN6720350

Hersteller Produkt- Nr.: koa0748

Dieses Colorimetric ELISA-Kit wurde entwickelt für die quantitative Messung von Human PRSS8.

Kurzübersicht für PRSS8 ELISA Kit (ABIN6720350)

Target: PRSS8 (Protease, serine, 8 (PRSS8))

Reaktivität: Human

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Detektionsbereich: 1.56 ng/mL - 100 ng/mL

Applikation: ELISA

Proben: Cell Culture Supernatant, Plasma (EDTA - heparin), Serum, Urine

Produktdetails PRSS8 ELISA Kit

Untere Nachweisgrenze: 1.56 ng/mL

Hersteller Produkt- Nr.: koa0748

Hersteller: Rockland

Verwendungszweck: Human Prostasin Sandwich ELISA Kit for Quantitative Detection

Marke: AccuSignal™

Analytische Methode: Quantitative

Spezifität:

Production: Natural and recombinant human Prostasin. There is no detectable cross-reactivity with other relevant proteins.

Sensitivität: < 50 pg/mL

Bestandteile:

• Antibody-coated 96-well plate
• Target Protein Standard
• Detection antibody
• Detection reagent
• Diluent buffers
• Wash buffers
• Substrate Solution
• Stop solutions
• Adhesive covers

Anwendungsinformationen

Applikationshinweise: Useful in Sandwich ELISA for Quantitative Detection of Antigen. Aliquot 0.1 mL per well of the 100 ng/mL, 50 ng/mL, 25 ng/mL, 12.5 ng/mL, 6.25 ng/mL, 3.12 ng/mL, 1.56 ng/mL human Prostasin standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of human cell culture supernates, serum, plasma(heparin, EDTA) or urine to each empty well. It is recommended that each human Prostasin standard solution and each sample be measured in duplicate.

Kommentare:

Standard: Expression system for standard: CHO, Immunogen sequence: A33-G319

Probenmenge: 100 μL

Plattentyp: Pre-coated

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store vials at 4°C prior to opening. Centrifuge product if not completely clear after standing at room temperature. This product is stable for 6 months at 4°C as an undiluted liquid. Dilute only prior to immediate use. For extended storage freeze at -20°C or below for 12 months. Avoid cycles of freezing and thawing.

Haltbarkeit: 12 months

Details zu PRSS8

Target: PRSS8 (Protease, serine, 8 (PRSS8))

Andere Bezeichnung: Prostasin

Hintergrund:

Synonyms: 2410039E18Rik, AI313909, C79772, CAP1, Channel Activating Protease 1, mCAP1, Prostasin, Prostasin heavy chain, protease, serine, 8, Prss8, PRSS8_HUMAN, Serine protease 8

Background: Prostasin is a protein that in humans is encoded by the PRSS8 gene. It is a single-copy gene and mapped to 16p11.2. Prostasin is likely to be the more physiologically relevant protease. This gene encodes a trypsinogen, which is a member of the trypsin family of serine proteases. Coexpression of Prostasin with either Xenopus or rat ENaC in Xenopus oocytes resulted in a 60 % to 80 % increase in amiloride-sensitive sodium currents, and that the addition of aprotinin, a serine protease inhibitor, completely prevented this activation. This enzyme is highly expressed in prostate epithelia and is one of several proteolytic enzymes found in seminal fluid. The proprotein is cleaved to produce a light chain and a heavy chain which are associated by a disulfide bond. It is active on peptide linkages involving the carboxyl group of lysine or arginine.

Gen-ID: 5652

NCBI Accession: NP_002764

UniProt: Q16651