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IGFBPI ELISA Kit

IGFBPI Reaktivität: Human Colorimetric Sandwich ELISA 31.2 pg/mL - 2000 pg/mL Cell Culture Supernatant, Plasma (EDTA - heparin), Serum, Urine
Produktnummer ABIN6720316
$545.00
Zzgl. Versandkosten $45.00
1 kit
Lieferung in 1 bis 2 Werktagen
  • Target Alle IGFBPI ELISA Kits anzeigen
    IGFBPI (Insulin-Like Growth Factor Binding Protein 1 (IGFBPI))
    Reaktivität
    • 6
    • 5
    • 3
    • 3
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Human
    Nachweismethode
    Colorimetric
    Methodentyp
    Sandwich ELISA
    Detektionsbereich
    31.2 pg/mL - 2000 pg/mL
    Untere Nachweisgrenze
    31.2 pg/mL
    Applikation
    ELISA
    Verwendungszweck
    Sandwich ELISA for Quantitative Detection of Antigen
    Proben
    Cell Culture Supernatant, Plasma (EDTA - heparin), Serum, Urine
    Analytische Methode
    Quantitative
    Produktmerkmale

    Synonyms: AFBP, hIGFBP-1, IBP1, IGF-binding protein 1, IGF-BP25, Insulin-like growth factor-binding protein 1, Placental protein 12, PP12

    Background: IGFBP1 is also known as amniotic fluid binding protein(AFBP), placental protein-12, alpha-pregnancy-associated endometrial globulin, growth hormone independent binding protein, binding protein-28, binding protein-26, and binding protein-25. The low-molecular weight insulin-like growth-factor binding protein(IGF-BP25) is synthesized by human liver, secretory endometrium and decidua, and is also present in human serum. It binds insulin-like growth factors IGF-I and IGF-II with high affinity, and is proposed to act as a paracrine regulator of cell growth. These IGF-binding proteins are expressed at different concentrations in different tissues and are thought to regulate the activity of IGF I and II. The gene is organized in four exons and spans 5.9 kb. The IBP-1 gene is a single copy gene, located on chromosome 7. The standard product used in this kit is recombinant human IGFBP-1, consisting of 234 amino acids with the molecular mass of 25KDa.

    Gene Name: IGFBP1

    Production: Natural and recombinant human IGFBP-1. There is no detectable cross-reactivity with other relevant proteins.

    Standard: Expression system for standard: NSO, Immunogen sequence: A26-N259

  • Applikationshinweise

    Application Note: Useful in Sandwich ELISA for Quantitative Detection of Antigen. Aliquot 0.1 mL per well of the 2000pg/mL,1000pg/mL, 500pg/mL, 250pg/mL, 125pg/mL, 62.5pg/mL, 31.2pg/mL human IGFBP-1 standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of human cell culture supernates, serum, plasma (heparin, EDTA) or urine to each empty well. It is recommended that each human IGFBP-1 standard solution and each sample be measured in duplicate.

    Blood Product Anticoagulant: Heparin Sodium

    ELISA Dilution: 31.2pg/mL-2000pg/mL

    Probenmenge
    100 μL
    Plattentyp
    Pre-coated
    Beschränkungen
    Nur für Forschungszwecke einsetzbar
  • Lagerung
    RT,4 °C,-20 °C
    Informationen zur Lagerung
    Store vials at 4°C prior to opening. Centrifuge product if not completely clear after standing at room temperature. This product is stable for 6 months at 4°C as an undiluted liquid. Dilute only prior to immediate use. For extended storage freeze at -20°C or below for 12 months. Avoid cycles of freezing and thawing.
  • Target Alle IGFBPI ELISA Kits anzeigen
    IGFBPI (Insulin-Like Growth Factor Binding Protein 1 (IGFBPI))
    Andere Bezeichnung
    IGFBP-1 (IGFBPI Produkte)
    Synonyme
    cb656, igfbp1, IGFBP-1, MGC68538, afbp, ibp1, pp12, igf-bp25, higfbp-1, IGFBP1, LOC100223208, LOC100305121, AFBP, IBP1, IGF-BP25, PP12, hIGFBP-1, IGFBA, insulin like growth factor binding protein 1, insulin-like growth factor binding protein 1a, insulin like growth factor binding protein 1 S homeolog, insulin like growth factor binding protein 1 L homeolog, insulin-like growth factor binding protein 1, IGFBP1, igfbp1a, igfbp1.S, igfbp1.L, igfbp1, LOC100305121, Igfbp1
    Gen-ID
    3484
    NCBI Accession
    NP_000587
    UniProt
    P08833
    Pathways
    Myometrial Relaxation and Contraction, ER-Nucleus Signaling, Growth Factor Binding
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