Resistin ELISA Kit

Produktnummer ABIN6720305

Hersteller Produkt- Nr.: koa0356

Dieses Colorimetric ELISA-Kit wurde entwickelt für die quantitative Messung von Maus Resistin.

Kurzübersicht für Resistin ELISA Kit (ABIN6720305)

Target: Resistin (RETN)

Reaktivität: Maus

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Detektionsbereich: 31.2 pg/mL - 2000 pg/mL

Applikation: ELISA

Proben: Cell Culture Supernatant, Plasma (heparin), Serum

Produktdetails Resistin ELISA Kit

Untere Nachweisgrenze: 31.2 pg/mL

Hersteller Produkt- Nr.: koa0356

Hersteller: Rockland

Verwendungszweck: Mouse Resistin Sandwich ELISA Kit for Quantitative Detection

Marke: AccuSignal™

Analytische Methode: Quantitative

Spezifität:

Production: Natural and recombinant mouse Resistin. There is no detectable cross-reactivity with other relevant proteins.

Sensitivität: < 2 pg/mL

Bestandteile:

• Antibody-coated 96-well plate
• Target Protein Standard
• Detection antibody
• Detection reagent
• Diluent buffers
• Wash buffers
• Substrate Solution
• Stop solutions
• Adhesive covers

Anwendungsinformationen

Applikationshinweise: Useful in Sandwich ELISA for Quantitative Detection of Antigen. Aliquot 0.1 mL per well of the 2000pg/mL, 1000pg/mL, 500pg/mL, 250pg/mL, 125pg/mL, 62.5pg/mL, 31.2pg/mL mouse Resistin standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of mouse cell culture supernates, serum or plasma(heparin) to each empty well. We recommend that each mouse Resistin standard solution and each sample is measured in duplicate.

Kommentare:

Standard: Expression system for standard: E.coli, Immunogen sequence: S21-S114

Probenmenge: 100 μL

Plattentyp: Pre-coated

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store vials at 4°C prior to opening. Centrifuge product if not completely clear after standing at room temperature. This product is stable for 6 months at 4°C as an undiluted liquid. Dilute only prior to immediate use. For extended storage freeze at -20°C or below for 12 months. Avoid cycles of freezing and thawing.

Haltbarkeit: 12 months

Details zu Resistin

Target: Resistin (RETN)

Andere Bezeichnung: Resistin

Hintergrund:

Synonyms: Adipose tissue specific secretory factor, Adipose tissue-specific secretory factor, ADSF, C/EBP epsilon regulated myeloid specific secreted cysteine rich protein, C/EBP epsilon regulated myeloid specific secreted cysteine rich protein precursor 1, Cysteine rich secreted protein A12 alpha like 2, Cysteine rich secreted protein FIZZ3, Cysteine-rich secreted protein A12-alpha-like 2, Cysteine-rich secreted protein FIZZ3, FIZZ3, Found in inflammatory zone 3, Resistin, Resistin delta2, RETN_HUMAN, RETN1, XCP 1, XCP1

Background: Resistin is an adipose-derived hormone postulated to link adiposity to insulin resistance. Type 2 diabetes, characterized by target-tissue resistance to insulin, is epidemic in industrialized societies and is strongly associated with obesity. Adipocytes secrete a unique signalling molecule, which called resistin (for resistance to insulin), that may be the hormone potentially links obesity to diabetes. Resistin is overexpressed in mouse adipose tissue of obese individuals and is likely to modulate insulin sensitivity. Resistin is, therefore, a candidate gene for insulin resistance. The standard product used in this kit is mouse Resistin with the molecular mass of 51-53KDa. The detected Resistin includes zymogen and active enzyme.

Gen-ID: 57264

NCBI Accession: NP_001191888

UniProt: Q5BMX4

Pathways: Feeding Behaviour, Smooth Muscle Cell Migration