REG4 ELISA Kit

Produktnummer ABIN6720301

Hersteller Produkt- Nr.: koa0765

Human REG4 ELISA-Kit Colorimetric-Assay zur Quantifizierung von Human REG4.

Kurzübersicht für REG4 ELISA Kit (ABIN6720301)

Target: REG4 (Regenerating Islet-Derived Family, Member 4 (REG4))

Reaktivität: Human

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Detektionsbereich: 156 pg/mL - 10000 pg/mL

Applikation: ELISA

Proben: Cell Culture Supernatant, Plasma (heparin), Serum

Produktdetails REG4 ELISA Kit

Untere Nachweisgrenze: 156 pg/mL

Hersteller Produkt- Nr.: koa0765

Hersteller: Rockland

Verwendungszweck: Human REG-4 Sandwich ELISA Kit for Quantitative Detection

Marke: AccuSignal™

Analytische Methode: Quantitative

Spezifität:

Production: Natural and recombinant human REG-4. There is no detectable cross-reactivity with other relevant proteins.

Sensitivität: < 10 pg/mL

Bestandteile:

• Antibody-coated 96-well plate
• Target Protein Standard
• Detection antibody
• Detection reagent
• Diluent buffers
• Wash buffers
• Substrate Solution
• Stop solutions
• Adhesive covers

Anwendungsinformationen

Applikationshinweise: Useful in Sandwich ELISA for Quantitative Detection of Antigen. Aliquot 0.1 mL per well of the 10,000pg/mL, 5000pg/mL, 2500pg/mL, 1250pg/mL, 625pg/mL, 312pg/mL, 156pg/mL human REG-4 standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of human cell culture supernates, serum or plasma(heparin) to each empty well. It is recommended that each human REG-4 standard solution and each sample be measured in duplicate.

Kommentare:

Standard: Expression system for standard: E.coli, Immunogen sequence: D23-P158

Probenmenge: 100 μL

Plattentyp: Pre-coated

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store vials at 4°C prior to opening. Centrifuge product if not completely clear after standing at room temperature. This product is stable for 6 months at 4°C as an undiluted liquid. Dilute only prior to immediate use. For extended storage freeze at -20°C or below for 12 months. Avoid cycles of freezing and thawing.

Haltbarkeit: 12 months

Details zu REG4

Target: REG4 (Regenerating Islet-Derived Family, Member 4 (REG4))

Andere Bezeichnung: REG-4

Hintergrund:

Synonyms: Gastrointestinal secretory protein, GISP, REG IV, REG like protein, REG-4, REG-like protein, Reg4, REG4_HUMAN, Regenerating gene type IV, Regenerating islet derived family member 4, Regenerating islet derived protein 4 precursor, Regenerating islet-derived protein 4, Regenerating islet-derived protein IV, REGIV, RELP

Background: Regenerating islet-derived protein 4 is a protein that in humans is encoded by the REG4 gene. It is mapped to 1p12. REG-4 gene contains 6 exons and that the exon structure is preserved among members of the REG gene family. This gene is able to maintain carbohydrate recognition activity in an acidic environment. REG-4 expression was significantly upregulated by inflammation and tissue injury associated with active Crohn disease and ulcerative colitis, and most of the colorectal tumors overexpressing REG-4 are mucinous tumors or neuroendocrine tumors.

Gen-ID: 83998

NCBI Accession: NP_001152824

UniProt: Q9BYZ8