LUM ELISA Kit

Produktnummer ABIN6720300

Produktdetails LUM ELISA Kit

Target: LUM (Lumican (LUM))

Reaktivität: Human

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Detektionsbereich: 156 pg/mL - 10000 pg/mL

Untere Nachweisgrenze: 156 pg/mL

Applikation: ELISA

Verwendungszweck: Sandwich ELISA for Quantitative Detection of Antigen

Proben: Cell Culture Supernatant, Plasma (heparin), Serum

Analytische Methode: Quantitative

Produktmerkmale:

Synonyms: Keratan sulfate proteoglycan lumican, KSPG Lumican, LDC, LUM, LUM_HUMAN, Lumican, Lumican precursor, SLRR2D

Background: Lumican, also known as LUM, is a protein which in humans is encoded by the LUM gene. This gene is a member of the small interstitial proteoglycan gene(SIPG) family, and it is a keratan sulfate proteoglycan which presents large quantities in the corneal stroma and in interstitial collagenous matrices of the heart, aorta, skeletal muscle, skin, and intervertebral discs. Lumican is mapped to 12q21.33, it interacts with collagen and limits growth of fibrils in diameter. In the cornea, Lumican not only interacts with collagen molecules to limit fibril growth, but also plays a critical role in the regular spacing of fibrils and acquisition of corneal transparency by virtue of its keratan sulfate-containing glycosaminoglycan side chains LDC.

Gene Name: LUM

Production: Natural and recombinant human Lumican. There is no detectable cross-reactivity with other relevant proteins.

Standard: Expression system for standard: NSO, Immunogen sequence: Q19-N338

Anwendungsinformationen

Applikationshinweise:

Application Note: Useful in Sandwich ELISA for Quantitative Detection of Antigen. Aliquot 0.1 mL per well of the 10,000pg/mL, 5000pg/mL, 2500pg/mL, 1250pg/mL, 625pg/mL, 312pg/mL, 156pg/mL human Lumican standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of human cell culture supernates, serum or plasma(heparin) to each empty well. It is recommended that each human Lumican standard solution and each sample be measured in duplicate.

Blood Product Anticoagulant: Heparin Sodium

ELISA Dilution: 156pg/mL-10,000pg/mL

Probenmenge: 100 μL

Plattentyp: Pre-coated

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Lagerung: RT,4 °C,-20 °C

Informationen zur Lagerung: Store vials at 4°C prior to opening. Centrifuge product if not completely clear after standing at room temperature. This product is stable for 6 months at 4°C as an undiluted liquid. Dilute only prior to immediate use. For extended storage freeze at -20°C or below for 12 months. Avoid cycles of freezing and thawing.

Details zu LUM

Target: LUM (Lumican (LUM))

Andere Bezeichnung: Lumican (LUM Produkte)

Synonyme: MGC64527 ELISA Kit, zgc:86661 ELISA Kit, ldc ELISA Kit, slrr2d ELISA Kit, DKFZp468L1116 ELISA Kit, LDC ELISA Kit, SLRR2D ELISA Kit, Ldc ELISA Kit, lumican ELISA Kit, lumican L homeolog ELISA Kit, LUM ELISA Kit, lum.L ELISA Kit, lum ELISA Kit, CpipJ_CPIJ010209 ELISA Kit, Lum ELISA Kit

Gen-ID: 4060

NCBI Accession: NP_002336

UniProt: P51884

Pathways: Glycosaminoglycan Metabolic Process