IGF2 ELISA Kit

Produktnummer ABIN6720286

Produktdetails IGF2 ELISA Kit

Target: IGF2 (Insulin-Like Growth Factor 2 (IGF2))

Reaktivität: Ratte

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Detektionsbereich: 62.5 pg/mL - 4000 pg/mL

Untere Nachweisgrenze: 62.5 pg/mL

Applikation: ELISA

Verwendungszweck: Sandwich ELISA for Quantitative Detection of Antigen

Proben: Cell Culture Supernatant, Plasma (EDTA - heparin), Serum

Analytische Methode: Quantitative

Produktmerkmale:

Synonyms: C11orf43, FLJ22066, FLJ44734, IGF 2, IGF II, IGF-II, IGF2, IGF2_HUMAN, IGFII, INSIGF, Insulin like Growth Factor 2, Insulin like growth factor 2 (somatomedin A), Insulin like growth factor II, Insulin like growth factor II precursor, Insulin like growth factor type 2, pp9974, Preptin, Putative insulin like growth factor II associated protein, Somatomedin A, Somatomedin-A

Background: Insulin-like growth factor II is also known as somatomedin A. IGF-2 is a member of the insulin family of polypeptide growth factors that is involved in development and growth. It is paternally expressed in the fetus and placenta. IGF-II is a mitogen for many cell types and an important modulator of muscle growth and differentiation. IGF-II gene is prevalently expressed during prenatal development and its gene activity is regulated by genomic imprinting, in that the allele inherited from the father is active and the allele inherited from the mother is inactive in most normal tissues. IGF-II appears to be induced by placental lactogen during prenatal development. It is a mediator of prolactin-induced alveologenesis, prolactin, IGF-2, and cyclin D1, all of which are overexpressed in breast cancers, are components of a developmental pathway in the mammary gland.

Gene Name: IGF2

Production: Natural and recombinant rat IGF-2. There is no detectable cross-reactivity with other relevant proteins.

Standard: Expression system for standard: E.coli, Immunogen sequence: A25-E91

Anwendungsinformationen

Applikationshinweise:

Application Note: Useful in Sandwich ELISA for Quantitative Detection of Antigen. Aliquot 0.1 mL per well of the 4000pg/mL, 2000pg/mL,1000pg/mL, 500pg/mL, 250pg/mL, 125pg/mL, 62.5pg/mL rat IGF-2 standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of rat cell culture supernates, serum or plasma(heparin, EDTA) to each empty well. It is recommended that each rat IGF-2 standard solution and each sample be measured in duplicate.

Blood Product Anticoagulant: Heparin Sodium

ELISA Dilution: 62.5pg/mL-4000pg/mL

Probenmenge: 100 μL

Plattentyp: Pre-coated

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Lagerung: RT,4 °C,-20 °C

Informationen zur Lagerung: Store vials at 4°C prior to opening. Centrifuge product if not completely clear after standing at room temperature. This product is stable for 6 months at 4°C as an undiluted liquid. Dilute only prior to immediate use. For extended storage freeze at -20°C or below for 12 months. Avoid cycles of freezing and thawing.

Details zu IGF2

Target: IGF2 (Insulin-Like Growth Factor 2 (IGF2))

Andere Bezeichnung: IGF-2 (IGF2 Produkte)

Synonyme: C11orf43 ELISA Kit, IGF-II ELISA Kit, PP9974 ELISA Kit, LOC100136550 ELISA Kit, igf2 ELISA Kit, igf2b ELISA Kit, wu:fc26e03 ELISA Kit, zgc:91781 ELISA Kit, igf-2 ELISA Kit, insigf ELISA Kit, pp9974 ELISA Kit, AL033362 ELISA Kit, Igf-2 ELISA Kit, Igf-II ELISA Kit, M6pr ELISA Kit, Mpr ELISA Kit, Peg2 ELISA Kit, IGFII ELISA Kit, RNIGF2 ELISA Kit, IGF-II-B ELISA Kit, igf2-a ELISA Kit, igf2-b ELISA Kit, insulin like growth factor 2 ELISA Kit, insulin-like growth factor II ELISA Kit, insulin-like growth factor 2a ELISA Kit, insulin like growth factor 2 S homeolog ELISA Kit, insulin-like growth factor 2 ELISA Kit, insulin-like growth factor 2 (somatomedin A) ELISA Kit, insulin like growth factor 2 L homeolog ELISA Kit, IGF2 ELISA Kit, LOC100136550 ELISA Kit, igf2a ELISA Kit, igf2.S ELISA Kit, Igf2 ELISA Kit, igf2.L ELISA Kit

Gen-ID: 24483

NCBI Accession: NP_001177091

UniProt: P01346

Pathways: Hormone Activity, Regulation of Hormone Metabolic Process, Regulation of Hormone Biosynthetic Process, Regulation of Carbohydrate Metabolic Process, Activated T Cell Proliferation