CCL13 ELISA Kit

Produktnummer ABIN6720261

Hersteller Produkt- Nr.: koa0480

Dieses Human CCL13 ELISA-Kit ist ein Colorimetric ELISA-Kit, das dafür entwickelt wurde, Human CCL13 zu quantifizieren.

Kurzübersicht für CCL13 ELISA Kit (ABIN6720261)

Target: CCL13 (Chemokine (C-C Motif) Ligand 13 (CCL13))

Reaktivität: Human

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Detektionsbereich: 7.8 pg/mL - 500 pg/mL

Applikation: ELISA

Proben: Cell Culture Supernatant, Plasma (EDTA - heparin), Serum

Produktdetails CCL13 ELISA Kit

Untere Nachweisgrenze: 7.8 pg/mL

Hersteller Produkt- Nr.: koa0480

Hersteller: Rockland

Verwendungszweck: Human CCL13 - MCP4 Sandwich ELISA Kit for Quantitative Detection

Marke: AccuSignal™

Analytische Methode: Quantitative

Spezifität:

Production: Natural and recombinant human CCL13. There is no detectable cross-reactivity with other relevant proteins.

Sensitivität: < 10 pg/mL

Bestandteile:

• Antibody-coated 96-well plate
• Target Protein Standard
• Detection antibody
• Detection reagent
• Diluent buffers
• Wash buffers
• Substrate Solution
• Stop solutions
• Adhesive covers

Anwendungsinformationen

Applikationshinweise: Useful in Sandwich ELISA for Quantitative Detection of Antigen. Aliquot 0.1 mL per well of the 500pg/mL, 250pg/mL, 125pg/mL, 62.5pg/mL, 31.3pg/mL, 15.6pg/mL, 7.8pg/mL human CCL13 standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of human cell culture supernates, serum or plasma(heparin, EDTA) to each empty well. We recommend that each human CCL13 standard solution and each sample is measured in duplicate.

Kommentare:

Standard: Expression system for standard: E.coli, Immunogen sequence: Q24-T98

Probenmenge: 100 μL

Plattentyp: Pre-coated

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store vials at 4°C prior to opening. Centrifuge product if not completely clear after standing at room temperature. This product is stable for 6 months at 4°C as an undiluted liquid. Dilute only prior to immediate use. For extended storage freeze at -20°C or below for 12 months. Avoid cycles of freezing and thawing.

Haltbarkeit: 12 months

Details zu CCL13

Target: CCL13 (Chemokine (C-C Motif) Ligand 13 (CCL13))

Andere Bezeichnung: CCL13/MCP4

Hintergrund:

Synonyms: C-C motif chemokine 13, CCL13, CCL13_HUMAN, Chemokine (C C motif) ligand 13, CK beta 10, CK-beta-10, CKb10, Macrophage/monocyte chemotactic protein 4, MCP-4, MGC17134, Monocyte chemoattractant protein 4, Monocyte chemotactic protein 4, NCC 1, NCC-1, NCC1, New CC chemokine 1, SCYA13, SCYL1, short chain, Small inducible cytokine A13, Small inducible cytokine A13 precursor, Small inducible cytokine subfamily A (Cys Cys) member 13, Small-inducible cytokine A13

Background: Chemokine(C-C motif) ligand 13(CCL13) is a small cytokine belonging to the CC chemokine family. Its gene is located on human chromosome 17 within a large cluster of other CC chemokines. CCL13 induces chemotaxis in monocytes, eosinophils, T lymphocytes, and basophils by binding cell surface G-protein linked chemokine receptors such as CCR2, CCR3 and CCR5. Activity of this chemokine has been implicated in allergic reactions such as asthma. CCL13 can be induced by the inflammatory cytokines interleukin-1 and TNF-alpha.

Gen-ID: 12978

NCBI Accession: NP_001032948

UniProt: P09581

Pathways: Regulation of Systemic Arterial Blood Pressure by Hormones, Phosphorylierungen bei SARS-CoV-2 Infektion