ANGPTL4 ELISA Kit

Produktnummer ABIN6720260

Hersteller Produkt- Nr.: koa0590

Dieses Colorimetric ELISA-Kit wurde entwickelt für die quantitative Messung von Human ANGPTL4.

Kurzübersicht für ANGPTL4 ELISA Kit (ABIN6720260)

Target: ANGPTL4 (Angiopoietin-Like 4 (ANGPTL4))

Reaktivität: Human

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Detektionsbereich: 625 pg/mL - 40000 pg/mL

Applikation: ELISA

Proben: Cell Culture Supernatant, Plasma (EDTA - heparin), Serum

Produktdetails ANGPTL4 ELISA Kit

Untere Nachweisgrenze: 625 pg/mL

Hersteller Produkt- Nr.: koa0590

Hersteller: Rockland

Verwendungszweck: Human ANGPTL4 Sandwich ELISA Kit for Quantitative Detection

Marke: AccuSignal™

Analytische Methode: Quantitative

Spezifität:

Production: Natural and recombinant human ANGPTL4. There is no detectable cross-reactivity with other relevant proteins.

Sensitivität: < 20 pg/mL

Bestandteile:

• Antibody-coated 96-well plate
• Target Protein Standard
• Detection antibody
• Detection reagent
• Diluent buffers
• Wash buffers
• Substrate Solution
• Stop solutions
• Adhesive covers

Anwendungsinformationen

Applikationshinweise: Useful in Sandwich ELISA for Quantitative Detection of Antigen. Aliquot 0.1 mL per well of the 40000pg/mL, 20000pg/mL, 10000pg/mL, 5000pg/mL, 2,500pg/mL, 1,250pg/mL, 625pg/mL human ANGPTL4 standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of human cell culture supernates, serum or plasma(heparin, EDTA) to each empty well. It is recommended that each human ANGPTL4 standard solution and each sample be measured in duplicate.

Kommentare:

Standard: Expression system for standard: NSO, Immunogen sequence: L165-S406

Probenmenge: 100 μL

Plattentyp: Pre-coated

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store vials at 4°C prior to opening. Centrifuge product if not completely clear after standing at room temperature. This product is stable for 6 months at 4°C as an undiluted liquid. Dilute only prior to immediate use. For extended storage freeze at -20°C or below for 12 months. Avoid cycles of freezing and thawing.

Haltbarkeit: 12 months

Details zu ANGPTL4

Target: ANGPTL4 (Angiopoietin-Like 4 (ANGPTL4))

Andere Bezeichnung: ANGPTL4

Hintergrund:

Synonyms: 425O18 1, Angiopoietin like 4, Angiopoietin related protein 4, ANGPT L2 , ANGPT L4, ANGPTL2, ANGPTL4 , Fasting induced adipose factor , FIAF, Fibrinogen/angiopoietin related protein, Hepatic fibrinogen/angiopoietin related protein , HFARP, Major histocompatibility complex region NG27, NL2, Peroxisome proliferator-activated receptor (PPAR) gamma induced angiopoietin related protein , PGAR, pp1158 , PPARG angiopoietin related protein , PSEC0166 , Secreted protein Bk89, Weakly similar to angiopoietin 1

Background: Angiopoietin-related protein 4(Angptl4) is a protein that in humans is encoded by the ANGPTL4 gene. This gene is a member of the angiopoietin/angiopoietin-like gene family and encodes a glycosylated, secreted protein with a fibrinogen C-terminal domain. This gene is induced under hypoxic conditions in endothelial cells and is the target of peroxisome proliferation activators. By radiation hybrid analysis, Angptl4 gene is mapped to 19p13.3. ANGPTL4 contributed to tumor growth and protected cells from anoikis, a form of programmed cell death induced when contact-dependent cells detach from the surrounding tissue matrix.

Gen-ID: 51129

NCBI Accession: NP_001034756

UniProt: Q9BY76

Pathways: Regulation of Lipid Metabolism by PPARalpha