Stanniocalcin 1 ELISA Kit

Produktnummer ABIN6720178

Hersteller Produkt- Nr.: koa0847

Human Stanniocalcin 1 ELISA-Kit Colorimetric-Assay zur Quantifizierung von Human Stanniocalcin 1.

Kurzübersicht für Stanniocalcin 1 ELISA Kit (ABIN6720178)

Target: Stanniocalcin 1 (STC1)

Reaktivität: Human

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Detektionsbereich: 31.2 pg/mL - 2000 pg/mL

Applikation: ELISA

Proben: Cell Culture Supernatant, Plasma (EDTA - heparin), Serum

Produktdetails Stanniocalcin 1 ELISA Kit

Untere Nachweisgrenze: 31.2 pg/mL

Hersteller Produkt- Nr.: koa0847

Hersteller: Rockland

Verwendungszweck: Human Stanniocalcin 1 - STC1 Sandwich ELISA Kit for Quantitative Detection

Marke: AccuSignal™

Analytische Methode: Quantitative

Spezifität:

Production: Natural and recombinant human STC1. There is no detectable cross-reactivity with other relevant proteins.

Sensitivität: < 10 pg/mL

Bestandteile:

• Antibody-coated 96-well plate
• Target Protein Standard
• Detection antibody
• Detection reagent
• Diluent buffers
• Wash buffers
• Substrate Solution
• Stop solutions
• Adhesive covers

Anwendungsinformationen

Applikationshinweise: Useful in Sandwich ELISA for Quantitative Detection of Antigen. Aliquot 0.1 mL per well of the 2000pg/mL,1000pg/mL, 500pg/mL, 250pg/mL, 125pg/mL, 62.5pg/mL, 31.2pg/mL human STC1 standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of human cell culture supernates, serum and plasma (heparin, EDTA) to each empty well. It is recommended that each human STC1 standard solution and each sample be measured in duplicate.

Kommentare:

Standard: Expression system for standard: NSO, Immunogen sequence: V34-A247

Probenmenge: 100 μL

Plattentyp: Pre-coated

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store vials at 4°C prior to opening. Centrifuge product if not completely clear after standing at room temperature. This product is stable for 6 months at 4°C as an undiluted liquid. Dilute only prior to immediate use. For extended storage freeze at -20°C or below for 12 months. Avoid cycles of freezing and thawing.

Haltbarkeit: 12 months

Details zu Stanniocalcin 1

Target: Stanniocalcin 1 (STC1)

Andere Bezeichnung: Stanniocalcin 1/STC1

Hintergrund:

Synonyms: Stanniocalcin, Stanniocalcin-1, STC, STC-1, STC1, STC1_HUMAN

Background: Stanniocalcin-1 is a glycoprotein which is encoded by the STC1 gene. This gene encodes a secreted, homodimeric glycoprotein that is expressed in a wide variety of tissues and may have autocrine or paracrine functions. Human Stanniocalcin-1 is a putative molecular biomarker of leukemic microenvironment and the only molecular function known up to date is a SUMO E3 ligase activity in the SUMOylation cycle. STC1 interacts with lots of proteins in the cytoplasm, mitochondria, endoplasmatic reticulum and dot-like fashion in the nucleus. The N-terminal region of STC1 is the function region which is responsible to establish the interaction with its partners, including SUMO1. Low resolution studies shows that STC1 is an anti-parallel homodimer in solution and the cystein 202 is responsible for the dimerization of this protein. All the 5 dissulfide bonds of human STC1 are conserved and have the same profile of fish STC.

Gen-ID: 6781

NCBI Accession: NP_003146

UniProt: P52823

Pathways: Hormone Activity