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GDF15 ELISA Kit

Dieses Colorimetric ELISA-Kit wurde entwickelt für die quantitative Messung von Human GDF15 in Cell Culture Supernatant, Plasma (EDTA - heparin) und Serum.
Rockland
Produktnummer ABIN6720167
Hersteller Produkt- Nr.: koa0453

Kurzübersicht für GDF15 ELISA Kit (ABIN6720167)

Target

Alle GDF15 ELISA Kits anzeigen
GDF15 (Growth Differentiation Factor 15 (GDF15))

Reaktivität

  • 7
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Human

Nachweismethode

Colorimetric

Methodentyp

Sandwich ELISA

Detektionsbereich

31.2 pg/mL - 2000 pg/mL

Applikation

ELISA

Proben

Cell Culture Supernatant, Plasma (EDTA - heparin), Serum
  • Untere Nachweisgrenze

    31.2 pg/mL

    Hersteller Produkt- Nr.

    koa0453

    Hersteller

    Rockland

    Verwendungszweck

    Human GDF15 Sandwich ELISA Kit for Quantitative Detection

    Marke

    AccuSignal™

    Analytische Methode

    Quantitative

    Spezifität

    Production: Natural and recombinant human GDF-15. There is no detectable cross-reactivity with other relevant proteins.

    Sensitivität

    < 10 pg/mL

    Bestandteile

    • Antibody-coated 96-well plate
    • Target Protein Standard
    • Detection antibody
    • Detection reagent
    • Diluent buffers
    • Wash buffers
    • Substrate Solution
    • Stop solutions
    • Adhesive covers
  • Applikationshinweise

    ELISA_Dilution: 31.2pg/mL-2000pg/mL

    Application_Note: Useful in Sandwich ELISA for Quantitative Detection of Antigen. Aliquot 0.1 mL per well of the 2000pg/mL,1000pg/mL, 500pg/mL, 250pg/mL, 125pg/mL, 62.5pg/mL, 31.2pg/mL human GDF-15 standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of human cell culture supernates, serum or plasma(heparin, EDTA) to each empty well. It is recommended that each human GDF-15 standard solution and each sample be measured in duplicate.

    Kommentare

    Standard: Expression system for standard: CHO, Immunogen sequence: A197-I308

    Probenmenge

    100 μL

    Plattentyp

    Pre-coated

    Beschränkungen

    Nur für Forschungszwecke einsetzbar
  • Lagerung

    4 °C,-20 °C

    Informationen zur Lagerung

    Store vials at 4°C prior to opening. Centrifuge product if not completely clear after standing at room temperature. This product is stable for 6 months at 4°C as an undiluted liquid. Dilute only prior to immediate use. For extended storage freeze at -20°C or below for 12 months. Avoid cycles of freezing and thawing.

    Haltbarkeit

    12 months
  • Target Alle GDF15 ELISA Kits anzeigen

    GDF15 (Growth Differentiation Factor 15 (GDF15))

    Andere Bezeichnung

    GDF-15

    Hintergrund

    Synonyms: Growth/differentiation factor 15, Macrophage inhibitory cytokine 1, MIC 1, NAG-1, NRG-1, NSAID-activated gene 1 protein, NSAID-regulated gene 1 protein, PDF, PLAB, Placental bone morphogenetic protein, Placental TGF-beta, Prostate differentiation factor, PROSTATE-DERIVED FACTOR, PTGFB

    Background: GDF-15(Growth differentiation factor 15),also known as TGF-PL, MIC-1, PDF, PLAB, and PTGFB, is a protein belonging to the transforming growth factor beta superfamily that has a role in regulating inflammatory and apoptotic pathways in injured tissues and during disease processes. Using FISH, the MIC1 gene is mapped to 19p13.2-p13.1. Its expression in liver can be significantly up-regulated in during injury of organs such as liver, kidney, heart and lung. GDF15 showed increased expression and secretion during erythroblast maturation. GDF15 functions as an anti-inflammatory cytokine by directly interfering with chemokine signaling and integrin activation.

    Gen-ID

    9518

    NCBI Accession

    NP_004855

    UniProt

    Q99988

    Pathways

    SARS-CoV-2 Protein Interaktom
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