CSF3R ELISA Kit

Produktnummer ABIN6720159

Hersteller Produkt- Nr.: koa0452

Dieses Human CSF3R ELISA-Kit ist ein Colorimetric ELISA-Kit, das dafür entwickelt wurde, Human CSF3R zu quantifizieren.

Kurzübersicht für CSF3R ELISA Kit (ABIN6720159)

Target: CSF3R (Colony Stimulating Factor 3 Receptor (Granulocyte) (CSF3R))

Reaktivität: Human

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Detektionsbereich: 31.2 pg/mL - 2000 pg/mL

Applikation: ELISA

Proben: Cell Culture Supernatant, Plasma (EDTA - heparin), Serum

Produktdetails CSF3R ELISA Kit

Untere Nachweisgrenze: 31.2 pg/mL

Hersteller Produkt- Nr.: koa0452

Hersteller: Rockland

Verwendungszweck: Human CSF3R - G-CSF R Sandwich ELISA Kit for Quantitative Detection

Marke: AccuSignal™

Analytische Methode: Quantitative

Spezifität:

Production: Natural and recombinant human CSF3R. There is no detectable cross-reactivity with other relevant proteins.

Sensitivität: < 10 pg/mL

Bestandteile:

• Antibody-coated 96-well plate
• Target Protein Standard
• Detection antibody
• Detection reagent
• Diluent buffers
• Wash buffers
• Substrate Solution
• Stop solutions
• Adhesive covers

Anwendungsinformationen

Applikationshinweise: Useful in Sandwich ELISA for Quantitative Detection of Antigen. Aliquot 0.1 mL per well of the 2000pg/mL, 1000pg/mL, 500pg/mL, 250pg/mL, 125pg/mL, 62.5pg/mL, 31.2pg/mL human CSF3R standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of human cell culture supernates, serum and plasma(heparin, EDTA) to each empty well. It is recommended that each human CSF3R standard solution and each sample be measured in duplicate.

Kommentare:

Standard: Expression system for standard: E.cNSO, Immunogen sequence: E25-P621

Probenmenge: 100 μL

Plattentyp: Pre-coated

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store vials at 4°C prior to opening. Centrifuge product if not completely clear after standing at room temperature. This product is stable for 6 months at 4°C as an undiluted liquid. Dilute only prior to immediate use. For extended storage freeze at -20°C or below for 12 months. Avoid cycles of freezing and thawing.

Haltbarkeit: 12 months

Details zu CSF3R

Target: CSF3R (Colony Stimulating Factor 3 Receptor (Granulocyte) (CSF3R))

Andere Bezeichnung: CSF3R/G-CSF R

Hintergrund:

Synonyms: CD 114, CD114, CD114 antigen, Colony stimulating factor 3 receptor (granulocyte), Colony stimulating factor 3 receptor, CSF 3R, CSF3R, CSF3R_HUMAN, Csfgr, G CSF R, G-CSF receptor, G-CSF-R, GCSFR, Granulocyte colony stimulating factor receptor, Granulocyte colony-stimulating factor receptor, OTTHUMP00000009703, OTTHUMP00000009704, OTTHUMP00000009705

Background: The granulocyte colony-stimulating factor receptor (G-CSF-R), also known as CD114 (Cluster of Differentiation 114), is a protein that in humans is encoded by the CSF3R gene. It is mapped to 1p35-p34.3. G-CSF-R is a cell-surface receptor for the granulocyte colony-stimulating factor (G-CSF). And the G-CSF-R is a transmembrane receptor that consists of an extracellular ligand-binding portion, a transmembrane domain, and the cytoplasmic portion that is responsible for signal transduction. In addition, GCSF-R ligand-binding is associated with dimerization of the receptor and signal transduction through proteins including Jak, Lyn, STAT, andErk1/2.

Gen-ID: 1441

NCBI Accession: NP_000751

UniProt: Q99062