CCL28 ELISA Kit

Produktnummer ABIN6720154

Produktdetails CCL28 ELISA Kit

Target: CCL28 (Chemokine (C-C Motif) Ligand 28 (CCL28))

Reaktivität: Human

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Detektionsbereich: 31.2 pg/mL - 2000 pg/mL

Untere Nachweisgrenze: 31.2 pg/mL

Applikation: ELISA

Verwendungszweck: Sandwich ELISA for Quantitative Detection of Antigen

Proben: Cell Culture Supernatant, Plasma (EDTA - heparin), Serum

Analytische Methode: Quantitative

Produktmerkmale:

Synonyms: CCK 1, CCK1, CCL 28, Chemokine (C-C motif) ligand 28, MEC, MGC71902, Mucosae associated epithelial chemokine, OTTHUMP0000022162, SCYA28, Small inducible cytokine A28, Small inducible cytokine A28 [Precursor], Small inducible cytokine subfamily A (Cys-Cys), member 28

Background: CCL28, also known as mucosae-associated epithelial chemokine (MEC), CCK1 and SCYA28, is a chemokine. It is mapped to 5p12. The tumor hypoxia promotes the recruitment of regulatory T (Treg) cells through induction of expression of the chemokine CCL28, which in turn promotes tumor tolerance and angiogenesis. CCL28 regulates the chemotaxis of cells that express the chemokine receptors CCR3 and CCR10. CCL28 has also been implicated in the migration of IgA-expressing cells to the mammary gland, salivary gland, intestine and other mucosal tissues. It has also been shown as a potential antimicrobial agent effective against certain pathogens, such as Gram negative and Gram positive bacteria and the fungus Candida albicans.

Gene Name: CCL28

Production: Natural and recombinant human CCL28. There is no detectable cross-reactivity with other relevant proteins.

Standard: Expression system for standard: E.coli, Immunogen sequence: I23-Y127

Anwendungsinformationen

Applikationshinweise:

Application Note: Useful in Sandwich ELISA for Quantitative Detection of Antigen. Aliquot 0.1 mL per well of the 2000pg/mL, 1000pg/mL, 500pg/mL, 250pg/mL, 125pg/mL, 62.5pg/mL, 31.2pg/mL human CCL28 standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of human cell culture supernates, serum and plasma(heparin, EDTA) to each empty well. It is recommended that each human CCL28 standard solution and each sample be measured in duplicate.

Blood Product Anticoagulant: Heparin Sodium

ELISA Dilution: 31.2pg/mL-2000pg/mL

Probenmenge: 100 μL

Plattentyp: Pre-coated

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Lagerung: RT,4 °C,-20 °C

Informationen zur Lagerung: Store vials at 4°C prior to opening. Centrifuge product if not completely clear after standing at room temperature. This product is stable for 6 months at 4°C as an undiluted liquid. Dilute only prior to immediate use. For extended storage freeze at -20°C or below for 12 months. Avoid cycles of freezing and thawing.

Details zu CCL28

Target: CCL28 (Chemokine (C-C Motif) Ligand 28 (CCL28))

Andere Bezeichnung: CCL28 (CCL28 Produkte)

Synonyme: Scya28 ELISA Kit, CCL28 ELISA Kit, ccl28 ELISA Kit, nrpb ELISA Kit, enc-1 ELISA Kit, pig10 ELISA Kit, Encr-3 ELISA Kit, klhl35 ELISA Kit, Xencr-3 ELISA Kit, tp53i10 ELISA Kit, LOC100303767 ELISA Kit, CCK1 ELISA Kit, MEC ELISA Kit, SCYA28 ELISA Kit, C-C motif chemokine ligand 28 ELISA Kit, ectodermal-neural cortex 1 (with BTB domain) ELISA Kit, C-C motif chemokine 28 ELISA Kit, chemokine (C-C motif) ligand 28 ELISA Kit, Ccl28 ELISA Kit, CCL28 ELISA Kit, ccl28 ELISA Kit, enc1 ELISA Kit, LOC100303767 ELISA Kit

Gen-ID: 65477

NCBI Accession: NP_001288802

UniProt: Q9NRJ3