CCL27 ELISA Kit

Produktnummer ABIN6720153

Hersteller Produkt- Nr.: koa0397

Dieses Human CCL27 ELISA-Kit ist ein Colorimetric ELISA-Kit, das dafür entwickelt wurde, Human CCL27 zu quantifizieren.

Kurzübersicht für CCL27 ELISA Kit (ABIN6720153)

Target: CCL27 (Chemokine (C-C Motif) Ligand 27 (CCL27))

Reaktivität: Human

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Detektionsbereich: 31.2 pg/mL - 2000 pg/mL

Applikation: ELISA

Proben: Cell Culture Supernatant, Plasma (EDTA - heparin), Serum

Produktdetails CCL27 ELISA Kit

Untere Nachweisgrenze: 31.2 pg/mL

Hersteller Produkt- Nr.: koa0397

Hersteller: Rockland

Verwendungszweck: Human CCL27 - CTACK Sandwich ELISA Kit for Quantitative Detection

Marke: AccuSignal™

Analytische Methode: Quantitative

Spezifität:

Production: Natural and recombinant human CCL27. There is no detectable cross-reactivity with other relevant proteins.

Sensitivität: < 10 pg/mL

Bestandteile:

• Antibody-coated 96-well plate
• Target Protein Standard
• Detection antibody
• Detection reagent
• Diluent buffers
• Wash buffers
• Substrate Solution
• Stop solutions
• Adhesive covers

Anwendungsinformationen

Applikationshinweise: Useful in Sandwich ELISA for Quantitative Detection of Antigen. Aliquot 0.1 mL per well of the 2000pg/mL, 1000pg/mL, 500pg/mL, 250pg/mL, 125pg/mL, 62.5pg/mL, 31.2pg/mL human CCL27 standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of human cell culture supernates, serum or plasma(heparin, EDTA) to each empty well. It is recommended that each human CCL27 standard solution and each sample be measured in duplicate.

Kommentare:

Standard: Expression system for standard: E.coli, Immunogen sequence: F25-G112

Probenmenge: 100 μL

Plattentyp: Pre-coated

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store vials at 4°C prior to opening. Centrifuge product if not completely clear after standing at room temperature. This product is stable for 6 months at 4°C as an undiluted liquid. Dilute only prior to immediate use. For extended storage freeze at -20°C or below for 12 months. Avoid cycles of freezing and thawing.

Haltbarkeit: 12 months

Details zu CCL27

Target: CCL27 (Chemokine (C-C Motif) Ligand 27 (CCL27))

Andere Bezeichnung: CCL27/CTACK

Hintergrund:

Synonyms: ALP, C-C motif chemokine 27, CC chemokine ILC, Ccl27, CCL27_HUMAN, CTACK, CTAK, Cutaneous T cell attracting chemokine, Cutaneous T-cell-attracting chemokine, ESkine, IL 11 R alpha locus chemokine, IL 11 Ralpha locus chemokine, IL-11 R-alpha-locus chemokine, ILC, Ligand 27, PESKY, SCYA27, Skinkine, Small inducible cytokine A27, Small inducible cytokine subfamily A (Cys-Cys), member 27, Small-inducible cytokine A27

Background: Chemokine(C-C motif) ligand 27(CCL27) is a small cytokine belonging to the CC chemokine family, it is also known under the names IL-11 R-alpha-locus chemokine(ILC), Skinkine, ESkine and Cutaneous T-cell-attracting chemokine(CTACK). This gene is mapped to 9p13.3. CCL27 is associated with homing of memory T lymphocytes to the skin, and plays a role in T cell-mediated inflammation of the skin. CCL27 is expressed in numerous tissues, including gonads, thymus, placenta and skin. It elicits its chemotactic effects by binding to the chemokine receptor CCR10.

Gen-ID: 10850

NCBI Accession: NP_006655

UniProt: Q9Y4X3