ESM1 ELISA Kit

Produktnummer ABIN6720142

Hersteller Produkt- Nr.: koa0445

Maus ESM1 ELISA Kit, Colorimetric Assay für die Quantifizierung von Maus ESM1.

Kurzübersicht für ESM1 ELISA Kit (ABIN6720142)

Target: ESM1 (Endothelial Cell-Specific Molecule 1 (ESM1))

Reaktivität: Maus

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Detektionsbereich: 23.4 pg/mL - 1500 pg/mL

Applikation: ELISA

Proben: Cell Culture Supernatant, Cell Lysate, Plasma (EDTA), Plasma (heparin), Serum

Produktdetails ESM1 ELISA Kit

Untere Nachweisgrenze: 23.4 pg/mL

Hersteller Produkt- Nr.: koa0445

Hersteller: Rockland

Verwendungszweck: Mouse ESM1 - Endocan Sandwich ELISA Kit for Quantitative Detection

Marke: AccuSignal™

Analytische Methode: Quantitative

Spezifität: Expression system for standard: NS0
Immunogen sequence: W20-R184

Kreuzreaktivität (Details): There is no detectable cross-reactivity with other relevant proteins.

Sensitivität: < 10 pg/mL

Bestandteile:

• Antibody-coated 96-well plate
• Target Protein Standard
• Detection antibody
• Detection reagent
• Diluent buffers
• Wash buffers
• Substrate Solution
• Stop solutions
• Adhesive covers

Benötigtes Material: Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional). Pipettes and pipette tips capable of precisely dispensing 0.5 μL through 1 mL volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500 mL graduated cylinders. Test tubes for dilution.

Anwendungsinformationen

Kommentare:

Capture antibody and Detection antibody: monoclonal antibody from rat, polyclonal antibody from goat

Probenmenge: 100 μL

Plattentyp: Pre-coated

Protokoll: Useful in Sandwich ELISA for Quantitative Detection of Antigen. Aliquot 0.1 mL per well of the 1500pg/mL, 750pg/mL, 419pg/mL, 187.5pg/mL, 93.7pg/mL, 46.8pg/mL, 23.4pg/mL mouse ESM1 standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of mouse cell culture supernates, serum or plasma(heparin, EDTA) to each empty well. It is recommended that each mouse ESM1 standard solution and each sample be measured in duplicate.

Testpräzision: Intra-Assay Precision (CV%): 6%, 6%, 7.7%
Inter-Assay Precision (CV%): 7%, 7.5%, 8.9%

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store vials at 4°C prior to opening. Centrifuge product if not completely clear after standing at room temperature. This product is stable for 6 months at 4°C as an undiluted liquid. Dilute only prior to immediate use. For extended storage freeze at -20°C or below for 12 months. Avoid cycles of freezing and thawing.

Haltbarkeit: 12 months

Details zu ESM1

Target: ESM1 (Endothelial Cell-Specific Molecule 1 (ESM1))

Andere Bezeichnung: ESM1/Endocan

Hintergrund:

Background: Endothelial cell-specific molecule 1, also known as Endocan, is a protein that in humans is encoded by the ESM1 gene. This gene encodes a secreted protein which is mainly expressed in the endothelial cells in human lung and kidney tissues. The expression of this gene is regulated by cytokines, suggesting that it may play a role in endothelium-dependent pathological disorders. The transcript contains multiple polyadenylation and mRNA instability signals. ESM-1 has been described as a specific biomarker of tip cells during neoangiogenesis by independent teams. Its expression has been shown to be increase in presence of pro-angiogenic growth factors such as VEGF (vascular endothelial growth factor) or FGF-2 (fibroblast growth factor 2).

Gene Full Name: endothelial cell-specific molecule 1

Gen-ID: 71690

NCBI Accession: NP_076101

UniProt: Q9QYY7

Pathways: Growth Factor Binding