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ICAM-3/CD50 ELISA Kit

Dieses Human ICAM-3/CD50 ELISA-Kit ist ein Colorimetric ELISA-Kit, das dafür entwickelt wurde, Human ICAM-3/CD50 zu quantifizieren.
Rockland
Produktnummer ABIN6720105
Hersteller Produkt- Nr.: koa0575

Kurzübersicht für ICAM-3/CD50 ELISA Kit (ABIN6720105)

Target

Alle ICAM-3/CD50 (ICAM3) ELISA Kits anzeigen
ICAM-3/CD50 (ICAM3) (Intercellular Adhesion Molecule 3 (ICAM3))

Reaktivität

  • 6
  • 1
  • 1
Human

Nachweismethode

Colorimetric

Methodentyp

Sandwich ELISA

Detektionsbereich

156 pg/mL - 10000 pg/mL

Applikation

ELISA

Proben

Cell Culture Supernatant, Plasma (EDTA - heparin), Serum
  • Untere Nachweisgrenze

    156 pg/mL

    Hersteller Produkt- Nr.

    koa0575

    Hersteller

    Rockland

    Verwendungszweck

    Human ICAM-3 Sandwich ELISA Kit for Quantitative Detection

    Marke

    AccuSignal™

    Analytische Methode

    Quantitative

    Spezifität

    Production: Natural and recombinant human ICAM-3. There is no detectable cross-reactivity with other relevant proteins.

    Sensitivität

    < 10 pg/mL

    Bestandteile

    • Antibody-coated 96-well plate
    • Target Protein Standard
    • Detection antibody
    • Detection reagent
    • Diluent buffers
    • Wash buffers
    • Substrate Solution
    • Stop solutions
    • Adhesive covers
  • Applikationshinweise

    Useful in Sandwich ELISA for Quantitative Detection of Antigen. Aliquot 0.1 mL per well of the 10000pg/mL, 5000pg/mL, 2500pg/mL, 1250pg/mL, 625pg/mL, 312pg/mL, 156pg/mL human ICAM-3 standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of human cell culture supernates, serum or plasma(heparin, EDTA) to each empty well. It is recommended that each human ICAM-3 standard solution and each sample be measured in duplicate.

    Kommentare

    Standard: Expression system for standard: NSO, Immunogen sequence: Q30-H485, homodimer

    Probenmenge

    100 μL

    Plattentyp

    Pre-coated

    Beschränkungen

    Nur für Forschungszwecke einsetzbar
  • Lagerung

    4 °C,-20 °C

    Informationen zur Lagerung

    Store vials at 4°C prior to opening. Centrifuge product if not completely clear after standing at room temperature. This product is stable for 6 months at 4°C as an undiluted liquid. Dilute only prior to immediate use. For extended storage freeze at -20°C or below for 12 months. Avoid cycles of freezing and thawing.

    Haltbarkeit

    12 months
  • Target Alle ICAM-3/CD50 (ICAM3) ELISA Kits anzeigen

    ICAM-3/CD50 (ICAM3) (Intercellular Adhesion Molecule 3 (ICAM3))

    Andere Bezeichnung

    ICAM-3

    Hintergrund

    Synonyms: CD50, CD50 antigen, CDW50, ICAM 3, ICAM R, ICAMR, Intercellular adhesion molecule 3, Intercellular adhesion molecule 3 precursor

    Background: Intercellular adhesion molecule 3(ICAM3) also known as CD50(Cluster of Differentiation 50), is a human gene. The protein encoded by this gene is a member of the intercellular adhesion molecule(ICAM) family. ICAM3 is closely related to ICAM1, consists of 5 immunoglobulin domains, and binds LFA1 through its 2 N-terminal domains. This protein is constitutively and abundantly expressed by all leucocytes and may be the most important ligand for LFA-1 in the initiation of the immune response. It functions not only as an adhesion molecule, but also as a potent signalling molecule. ICAM3 has been shown to interact with EZR and Moesin.

    Gen-ID

    3385

    NCBI Accession

    NP_002153

    UniProt

    P32942
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