DLL4 ELISA Kit

Produktnummer ABIN6720083

Hersteller Produkt- Nr.: koa0753

Dieses Colorimetric ELISA-Kit wurde entwickelt für die quantitative Messung von Maus DLL4.

Kurzübersicht für DLL4 ELISA Kit (ABIN6720083)

Target: DLL4 (delta-Like 4 (DLL4))

Reaktivität: Maus

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Detektionsbereich: 156 pg/mL - 10000 pg/mL

Applikation: ELISA

Proben: Cell Culture Supernatant, Plasma (EDTA - heparin), Serum

Produktdetails DLL4 ELISA Kit

Untere Nachweisgrenze: 156 pg/mL

Hersteller Produkt- Nr.: koa0753

Hersteller: Rockland

Verwendungszweck: Mouse DLL4 Sandwich ELISA Kit for Quantitative Detection

Marke: AccuSignal™

Analytische Methode: Quantitative

Spezifität:

Production: Natural and recombinant mouse DLL4. There is no detectable cross-reactivity with other relevant proteins.

Sensitivität: < 10 pg/mL

Bestandteile:

• Antibody-coated 96-well plate
• Target Protein Standard
• Detection antibody
• Detection reagent
• Diluent buffers
• Wash buffers
• Substrate Solution
• Stop solutions
• Adhesive covers

Anwendungsinformationen

Applikationshinweise: Useful in Sandwich ELISA for Quantitative Detection of Antigen. Aliquot 0.1 mL per well of the 10,000pg/mL, 5000pg/mL, 2500pg/mL, 1250pg/mL, 625pg/mL, 312pg/mL, 156pg/mL mouse DLL4 standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of mouse cell culture supernates, serum or plasma(heparin, EDTA) to each empty well. It is recommended that each mouse DLL4 standard solution and each sample be measured in duplicate.

Kommentare:

Standard: Expression system for standard: NSO, Immunogen sequence: S28-P525

Probenmenge: 100 μL

Plattentyp: Pre-coated

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store vials at 4°C prior to opening. Centrifuge product if not completely clear after standing at room temperature. This product is stable for 6 months at 4°C as an undiluted liquid. Dilute only prior to immediate use. For extended storage freeze at -20°C or below for 12 months. Avoid cycles of freezing and thawing.

Haltbarkeit: 12 months

Details zu DLL4

Target: DLL4 (delta-Like 4 (DLL4))

Andere Bezeichnung: DLL4

Hintergrund:

Synonyms: Delta 4, delta 4 precursor, Delta ligand 4, delta ligand 4 precursor, Delta like 4, Delta like 4 homolog, Delta like 4 protein, Delta like protein 4, Delta-like 4 (Drosophila), Delta-like protein 4, Delta4, DLL 4, DLL4, DLL4_HUMAN, Drosophila Delta homolog 4, hdelta2, Homeobox protein DLL-4, MGC126344, Notch ligand delta 2, Notch ligand DLL4, notch ligand DLL4 precursor, XDLL-4

Background: Delta like ligand 4 is a protein that in humans is encoded by the DLL4 gene. It is mapped to 15q15.1. This gene is a homolog of the Drosophila delta gene. DLL4 is a transmembrane ligand for Notch receptors that shows restricted expression to endothelial cells (ECs), in particular to arteries and capillaries, and is involved in vascular development, and it also appeared to be a major trigger of Notch receptor activities previously implicated in arterial and vascular development. Mouse DLL4 could activate mouse Notch1 and mouse Notch4. DLL4 acts as a negative regulator of tumor angiogenesis, its blockade results in the striking uncoupling of tumor growth from vessel density, presenting a novel therapeutic approach even for tumors resistant to anti-VEGF therapies. In addition to it, this gene also plays an important role in promoting Th17 effector activity during mycobacterial challenge.

Gen-ID: 54485

NCBI Accession: NP_062327

UniProt: Q9JI71

Pathways: Notch Signalweg