CCL19 ELISA Kit

Produktnummer ABIN6720039

Hersteller Produkt- Nr.: koa0264

Produktdetails CCL19 ELISA Kit

Target: CCL19 (Chemokine (C-C Motif) Ligand 19 (CCL19))

Reaktivität: Human

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Detektionsbereich: 15.6 pg/mL - 1000 pg/mL

Untere Nachweisgrenze: 15.6 pg/mL

Applikation: ELISA

Hersteller Produkt- Nr.: koa0264

Hersteller: Rockland

Verwendungszweck: Human CCL19 - MIP-3 beta Sandwich ELISA Kit for Quantitative Detection

Marke: AccuSignal™

Proben: Cell Culture Supernatant, Plasma (EDTA - heparin), Serum

Analytische Methode: Quantitative

Spezifität:

Production: Natural and recombinant human CCL19. There is no detectable cross-reactivity with other relevant proteins.

Sensitivität: < 10 pg/mL

Bestandteile:

• Antibody-coated 96-well plate
• Target Protein Standard
• Detection antibody
• Detection reagent
• Diluent buffers
• Wash buffers
• Substrate Solution
• Stop solutions
• Adhesive covers

Anwendungsinformationen

Applikationshinweise: Useful in Sandwich ELISA for Quantitative Detection of Antigen. Aliquot 0.1 mL per well of the 1000pg/mL, 500pg/mL, 250pg/mL, 125pg/mL, 62.5pg/mL, 31.2pg/mL, 15.6pg/mL human CCL19 standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of human cell culture supernates, serum or plasma(heparin, EDTA) to each empty well. It is recommended that each human CCL19 standard solution and each sample be measured in duplicate.

Kommentare:

Standard: Expression system for standard: E.coli, Immunogen sequence:  G22-S98

Probenmenge: 100 μL

Plattentyp: Pre-coated

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store vials at 4°C prior to opening. Centrifuge product if not completely clear after standing at room temperature. This product is stable for 6 months at 4°C as an undiluted liquid. Dilute only prior to immediate use. For extended storage freeze at -20°C or below for 12 months. Avoid cycles of freezing and thawing.

Haltbarkeit: 12 months

Details zu CCL19

Target: CCL19 (Chemokine (C-C Motif) Ligand 19 (CCL19))

Andere Bezeichnung: CCL19/MIP-3 beta (CCL19 Produkte)

Hintergrund:

Synonyms: Beta chemokine exodus 3, Beta-chemokine exodus-3, C C chemokine ligand 19, C-C motif chemokine 19, CC chemokine ligand 19, CCL19_HUMAN, Chemokine ligand 19, Chemokine ligand 19, Chemokine CC Motif Ligand 19, CK beta 11, CK beta-11, CKb11, EBI1 ligand chemokine, ELC, Epstein-Barr virus-induced molecule 1 ligand chemokine, Exodus 3, Macrophage inflammatory protein 3 beta, MIP 3 beta, MIP-3-beta, MIP-3B, MIP3 beta, MIP3B, SCYA19, Small inducible cytokine A19, Small-inducible cytokine A19

Background: Chemokine(C-C motif) ligand 19(CCL19) is a small cytokine belonging to the CC chemokine family that is also known as EBI1 ligand chemokine(ELC) and macrophage inflammatory protein-3-beta (MIP-3-beta). Using PCR of human-rodent hybrids, the CCL19 gene was map to human chromosome 9p13, and the CCL19 gene is not part of the CC chemokine gene cluster on chromosome 17. The cytokine encoded by this gene may play a role in normal lymphocyte recirculation and homing. It also plays an important role in trafficking of T cells in thymus, and in T cell and B cell migration to secondary lymphoid organs.

Gen-ID: 6363

NCBI Accession: NP_006265

UniProt: Q6IBD6

Pathways: Positive Regulation of Immune Effector Process