CCL14 ELISA Kit

Produktnummer ABIN6720037

Hersteller Produkt- Nr.: koa0643

Human CCL14 ELISA-Kit Colorimetric-Assay zur Quantifizierung von Human CCL14.

Kurzübersicht für CCL14 ELISA Kit (ABIN6720037)

Target: CCL14 (Chemokine (C-C Motif) Ligand 14 (CCL14))

Reaktivität: Human

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Detektionsbereich: 15.6 pg/mL - 1000 pg/mL

Applikation: ELISA

Proben: Cell Culture Supernatant, Plasma (EDTA - heparin), Serum

Produktdetails CCL14 ELISA Kit

Untere Nachweisgrenze: 15.6 pg/mL

Hersteller Produkt- Nr.: koa0643

Hersteller: Rockland

Verwendungszweck: Human CCL14 - HCC-1 Sandwich ELISA Kit for Quantitative Detection

Marke: AccuSignal™

Analytische Methode: Quantitative

Spezifität:

Production: Natural and recombinant human CCL14. There is no detectable cross-reactivity with other relevant proteins.

Sensitivität: < 10 pg/mL

Bestandteile:

• Antibody-coated 96-well plate
• Target Protein Standard
• Detection antibody
• Detection reagent
• Diluent buffers
• Wash buffers
• Substrate Solution
• Stop solutions
• Adhesive covers

Anwendungsinformationen

Applikationshinweise: Useful in Sandwich ELISA for Quantitative Detection of Antigen. Aliquot 0.1 mL per well of the 1000pg/mL, 500pg/mL, 250pg/mL, 125pg/mL, 62.5pg/mL, 31.3pg/mL, 15.6pg/mL human CCL14 standard solutions into the pre-coated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of human cell culture supernates, serum or plasma(heparin, EDTA) to each empty well. We recommend that each human CCL14 standard solution and each sample is measured in duplicate.

Kommentare:

Standard: Expression system for standard: E.coli, Immunogen sequence: T20-N93

Probenmenge: 100 μL

Plattentyp: Pre-coated

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store vials at 4°C prior to opening. Centrifuge product if not completely clear after standing at room temperature. This product is stable for 6 months at 4°C as an undiluted liquid. Dilute only prior to immediate use. For extended storage freeze at -20°C or below for 12 months. Avoid cycles of freezing and thawing.

Haltbarkeit: 12 months

Details zu CCL14

Target: CCL14 (Chemokine (C-C Motif) Ligand 14 (CCL14))

Andere Bezeichnung: CCL14/HCC-1

Hintergrund:

Synonyms: C-C motif chemokine 14,CC-1, CC-3, Chemokine (C-C motif) ligand 14, Chemokine CC-1/CC-3, Chemokine CC-3, CKB1, HCC-1, HCC-1(1-74), HCC-1/HCC-3, HCC-3, MCIF, NCC-2, NCC2, New CC chemokine 2, SCYA14, SCYL2, Small inducible cytokine subfamily A (Cys-Cys) member 14, Small-inducible cytokine A14, SY14

Background: Chemokine(C-C motif) ligand 14(CCL14) is a small cytokine belonging to the CC chemokine family. It is also commonly known as HCC-1. It is produced as a protein precursor that is processed to generate a mature active protein containing 74 amino acids that and is 46 % identical in amino acid composition to CCL3 and CCL4. This chemokine is expressed in various tissues including spleen, bone marrow, liver, muscle, and gut. CCL14 activates monocytes, but does not induce their chemotaxis. In addition, HCC-1 enhanced the proliferation of CD34+ myeloid progenitor cells. It was as effective as MIP-1 alpha, but about 100-fold less potent. Human CCL14 is located on chromosome 17 within a cluster of other chemokines belonging to the CC family.

Gen-ID: 6358

NCBI Accession: NP_116738

UniProt: Q16627