APCS ELISA Kit

Produktnummer ABIN6720025

Hersteller Produkt- Nr.: koa0706

Ratte APCS ELISA-Kit Colorimetric-Assay zur Quantifizierung von Ratte APCS.

Kurzübersicht für APCS ELISA Kit (ABIN6720025)

Target: APCS (Amyloid P Component, Serum (APCS))

Reaktivität: Ratte

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Detektionsbereich: 1.56 ng/mL - 100 ng/mL

Applikation: ELISA

Proben: Cell Culture Supernatant, Plasma (EDTA - heparin), Serum

Produktdetails APCS ELISA Kit

Untere Nachweisgrenze: 1.56 ng/mL

Hersteller Produkt- Nr.: koa0706

Hersteller: Rockland

Verwendungszweck: Rat SAP - PTX2 Sandwich ELISA Kit for Quantitative Detection

Marke: AccuSignal™

Analytische Methode: Quantitative

Spezifität:

Production: Natural and recombinant rat SAP. There is no detectable cross-reactivity with other relevant proteins.

Sensitivität: < 50 pg/mL

Bestandteile:

• Antibody-coated 96-well plate
• Target Protein Standard
• Detection antibody
• Detection reagent
• Diluent buffers
• Wash buffers
• Substrate Solution
• Stop solutions
• Adhesive covers

Anwendungsinformationen

Applikationshinweise:

ELISA_Dilution: 1.56 ng/mL-100 ng/mL

Application_Note: Useful in Sandwich ELISA for Quantitative Detection of Antigen. Aliquot 0.1 mL per well of the 100 ng/mL, 50 ng/mL, 25 ng/mL, 1.25 ng/mL, 6.25 ng/mL, 3.12 ng/mL, 1.56 ng/mL rat SAP standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of rat cell culture supernates, serum or plasma(heparin, EDTA) to each empty well. We recommend that each rat SAP standard solution and each sample is measured in duplicate.

Kommentare:

Standard: Expression system for standard: NSO, Immunogen sequence: Q21-S228

Probenmenge: 100 μL

Plattentyp: Pre-coated

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store vials at 4°C prior to opening. Centrifuge product if not completely clear after standing at room temperature. This product is stable for 6 months at 4°C as an undiluted liquid. Dilute only prior to immediate use. For extended storage freeze at -20°C or below for 12 months. Avoid cycles of freezing and thawing.

Haltbarkeit: 12 months

Details zu APCS

Target: APCS (Amyloid P Component, Serum (APCS))

Andere Bezeichnung: SAP/PTX2

Hintergrund:

Synonyms: 9.5S alpha-1-glycoprotein, APCS, SAMP_HUMAN, SAP, Serum amyloid P-component(1-203)

Background: Amyloid P component, serum(SAP), also known as PTX2 or APCS, is the identical serum form of amyloid P component(AP), a 25 kDa pentameric protein first identified as the pentagonal constituent of in vivo pathological deposits called "amyloid". It belongs to the pentraxins family, characterized by calcium dependent ligand binding and distinctive flattened beta-jellyroll structure similar to that of the legume lectins. This gene is mapped to 1q23.2. The binding of the encoded protein to proteins in the pathological amyloid cross-beta fold suggests its possible role as a chaperone. This protein is also thought to control the degradation of chromatin. It has been demonstrated that this protein binds to apoptotic cells at an early stage, which raises the possibility that it is involved in dealing with apoptotic cells in vivo.

Gen-ID: 29339

NCBI Accession: NP_058866

UniProt: P23680