KNG1 ELISA Kit

Produktnummer ABIN6720011

Hersteller Produkt- Nr.: koa0663

Dieses Colorimetric ELISA-Kit wurde entwickelt für die quantitative Messung von Human KNG1.

Kurzübersicht für KNG1 ELISA Kit (ABIN6720011)

Target: KNG1 (Kininogen 1 (KNG1))

Reaktivität: Human

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Detektionsbereich: 0.78 ng/mL - 50 ng/mL

Applikation: ELISA

Proben: Cell Culture Supernatant, Plasma (EDTA - heparin), Serum

Produktdetails KNG1 ELISA Kit

Untere Nachweisgrenze: 0.78 ng/mL

Hersteller Produkt- Nr.: koa0663

Hersteller: Rockland

Verwendungszweck: Human Kininogen-1 - KNG1 Sandwich ELISA Kit for Quantitative Detection

Marke: AccuSignal™

Analytische Methode: Quantitative

Spezifität:

Production: Natural and recombinant human Kininogen-1. There is no detectable cross-reactivity with other relevant proteins.

Sensitivität: < 20 pg/mL

Bestandteile:

• Antibody-coated 96-well plate
• Target Protein Standard
• Detection antibody
• Detection reagent
• Diluent buffers
• Wash buffers
• Substrate Solution
• Stop solutions
• Adhesive covers

Anwendungsinformationen

Applikationshinweise: Useful in Sandwich ELISA for Quantitative Detection of Antigen. Aliquot 0.1 mL per well of the 50 ng/mL, 25 ng/mL, 12.5 ng/mL, 6.25 ng/mL, 3.12 ng/mL, 1.56 ng/mL, 0.78 ng/mL human Kininogen-1 standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of human cell culture supernates, serum or plasma(heparin, EDTA) to each empty well. We recommend that each human Kininogen-1 standard solution and each sample is measured in duplicate.

Kommentare:

Standard: Expression system for standard: NSO, Immunogen sequence: Q19-S644

Probenmenge: 100 μL

Plattentyp: Pre-coated

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store vials at 4°C prior to opening. Centrifuge product if not completely clear after standing at room temperature. This product is stable for 6 months at 4°C as an undiluted liquid. Dilute only prior to immediate use. For extended storage freeze at -20°C or below for 12 months. Avoid cycles of freezing and thawing.

Haltbarkeit: 12 months

Details zu KNG1

Target: KNG1 (Kininogen 1 (KNG1))

Andere Bezeichnung: Kininogen-1/KNG1

Hintergrund:

Synonyms: Alpha-2-thiol proteinase inhibitor, BDK, BK, BK, included, bradykinin, bradykinin, included, Fitzgerald factor, Fitzgerald factor, included, Flaujeac factor, included, High molecular weight kininogen, High molecular weight kininogen, included, HMWK, HMWK, included, Ile-Ser-Bradykinin, Kallidin I, Kallidin II, kininogen 1, KNG, KNG1, KNG1_HUMAN, LMWK, included, Low molecular weight growth-promoting factor, Low molecular weight kininogen, included, Williams factor, included, Williams-Fitzgerald-Flaujeac factor

Background: Kininogen-1 (KNG1), also known as alpha-2-thiol proteinase inhibitor, Williams-Fitzgerald-Flaujeac factor or the HMWK-kallikrein factor, is a protein that in humans is encoded by the KNG1 gene. It is mapped to 3q27.3. The KNG1 gene uses alternative splicing to generate two different proteins - high - molecular - weight kininogen (HMWK) and low - molecular- weight kininogen (LMWK). HMWK is essential for blood coagulation and assembly of the kallikrein-kinin system. Also, bradykinin, a peptide causing numerous physiological effects, is released from HMWK. In contrast to HMWK, LMWK is not involved in blood coagulation. In addition to that, KNG1 is a constituent of the blood coagulation system as well as the kinin-kallikrein system.

Gen-ID: 3827

NCBI Accession: NP_000884

UniProt: P01042

Pathways: ACE Inhibitor Pathway, Glycosaminoglycan Metabolic Process