Fibronectin ELISA Kit

Produktnummer ABIN6720001

Hersteller Produkt- Nr.: koa0169

Human Fibronectin ELISA-Kit Colorimetric-Assay zur Quantifizierung von Human Fibronectin.

Kurzübersicht für Fibronectin ELISA Kit (ABIN6720001)

Target: Fibronectin

Reaktivität: Human

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Detektionsbereich: 156 pg/mL - 10000 pg/mL

Applikation: ELISA

Proben: Cell Culture Supernatant, Plasma (EDTA - heparin - citrate), Serum

Produktdetails Fibronectin ELISA Kit

Untere Nachweisgrenze: 156 pg/mL

Hersteller Produkt- Nr.: koa0169

Hersteller: Rockland

Verwendungszweck: Human Fibronectin Sandwich ELISA Kit for Quantitative Detection

Marke: AccuSignal™

Analytische Methode: Quantitative

Spezifität:

Production: Natural human Fibronectin. There is no detectable cross-reactivity with other relevant proteins.

Sensitivität: < 10 pg/mL

Bestandteile:

• Antibody-coated 96-well plate
• Target Protein Standard
• Detection antibody
• Detection reagent
• Diluent buffers
• Wash buffers
• Substrate Solution
• Stop solutions
• Adhesive covers

Anwendungsinformationen

Applikationshinweise: Useful in Sandwich ELISA for Quantitative Detection of Antigen. Aliquot 0.1 mL per well of the 10000pg/mL, 5000pg/mL, 2500pg/mL, 1250pg/mL, 625pg/mL, 312pg/mL, 156pg/mL human Fibronectin standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of human cell culture supernates, serum or plasma(heparin, EDTA, citrate) to each empty well. It is recommended that each human Fibronectin standard solution and each sample be measured in duplicate.

Kommentare:

Standard: From Plasma

Probenmenge: 100 μL

Plattentyp: Pre-coated

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store vials at 4°C prior to opening. Centrifuge product if not completely clear after standing at room temperature. This product is stable for 6 months at 4°C as an undiluted liquid. Dilute only prior to immediate use. For extended storage freeze at -20°C or below for 12 months. Avoid cycles of freezing and thawing.

Haltbarkeit: 12 months

Details zu Fibronectin

Target: Fibronectin

Hintergrund:

Synonyms: CIG, Cold insoluble globulin, Cold-insoluble globulin, Fibronectin 1, FINC, FINC_HUMAN, FN, FN1, FNZ, Migration stimulating factor, MSF, Ugl-Y3

Background: Fibronectin(FN) also known as LETS, is identified on the surfFN of fibroblasts by labeling with radioactive compounds or specific antibodies. Fibronectin is a 430,000-dalton dimeric glycoprotein that exists in 2 forms, termed cellular and plasma fibronectin. Cellular and plasma fibronectins are heterodimers consisting of similar but not identical polypeptides. These two forms of FN differ in biologic activity. Fibronectins bind cell surfFNs and various compounds including collagen, fibrin, heparin, DNA, and actin. Because fibronectin stimulates endocytosis in several systems and promotes the clearance of particulate material from the circulation, it could function in the clearance of C1q-coated material such as immune complexes or cellular debris. Fibronectins are involved in cell adhesion, cell motility, opsonization, would healing, and maintenance of cell shape. LETS, encoded on chromosome 8, is responsible for the LETS protein expression in humans. Because LETS has been implicated in tumorigenicity and cellular transformation, it is of interest that rearrangement or modifications in the number of chromosome 8 have been associated with certain forms of cancer. The standard used in this kit is isolated from human plasma with the molecular mass of 200-250KDa.

Gen-ID: 2335

NCBI Accession: NP_001293058

UniProt: P02751