VEGF ELISA Kit

Produktnummer ABIN6719993

Produktdetails VEGF ELISA Kit

Target: VEGF (Vascular Endothelial Growth Factor (VEGF))

Reaktivität: Maus

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Detektionsbereich: 15.6 pg/mL - 1000 pg/mL

Untere Nachweisgrenze: 15.6 pg/mL

Applikation: ELISA

Verwendungszweck: Sandwich ELISA for Quantitative Detection of Antigen

Proben: Cell Culture Supernatant, Plasma (EDTA - heparin - citrate), Serum

Analytische Methode: Quantitative

Produktmerkmale:

Synonyms: MGC70609, MVCD1, Vascular endothelial growth factor, Vascular endothelial growth factor A, Vascular permeability factor, VEGF A, VEGFA, VPF

Background: Vascular permeability factor/vascular endothelial growth factor(VPF/VEGF), a potent cytokine expressed by most malignant tumors, has critical roles in vasculogenesis and both physiological and pathological angiogenesis. VEGF produced by tumor cells potently stimulates endothelial cell proliferation and angiogenesis and plays a key role in the pathophysiology of several neoplasias. VEGF may also play a pivotal role in mediating the development and progression of diabetic retinopathy. VEGF, a major regulator of angiogenesis, binds to two receptor tyrosine kinases, KDR/Flk-1 and Flt-1.The VEGF gene is mapped by fluorescence in situ hybridization to chromosome 6p12. The standard product used in this kit is recombinant mouse VEGF164, a 42KDa dimer.

Gene Name: VEGFA

Production: This kit recognizes both the 164 and 120 amino acid residue forms of mouse VEGF. There is no detectable cross-reactivity with other relevant proteins.

Standard: Expression system for standard: sf21, Immunogen sequence: A27-R190

Anwendungsinformationen

Applikationshinweise:

Application Note: Useful in Sandwich ELISA for Quantitative Detection of Antigen. Aliquot 0.1 mL per well of the 1000pg/mL, 500pg/mL, 250pg/mL, 125pg/mL, 62.5pg/mL, 31.3pg/mL, 15.6pg/mL mouse VEGF standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of mouse cell culture supernates, serum or plasma(heparin, EDTA, citrate) to each empty well. We recommend that each mouse VEGF standard solution and each sample is measured in duplicate.

Blood Product Anticoagulant: Heparin Sodium

ELISA Dilution: 15.6pg/mL-1000pg/mL

Other Performance Data: DOE: 2-8° C- 3/29/18 -20° C - 9/29/18

Probenmenge: 100 μL

Plattentyp: Pre-coated

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Lagerung: RT,4 °C,-20 °C

Informationen zur Lagerung: Store vials at 4°C prior to opening. Centrifuge product if not completely clear after standing at room temperature. This product is stable for 6 months at 4°C as an undiluted liquid. Dilute only prior to immediate use. For extended storage freeze at -20°C or below for 12 months. Avoid cycles of freezing and thawing.

Details zu VEGF

Target: VEGF (Vascular Endothelial Growth Factor (VEGF))

Andere Bezeichnung: VEGF (VEGF Produkte)

Synonyme: MVCD1 ELISA Kit, VEGF ELISA Kit, VPF ELISA Kit, Vegf ELISA Kit, Vegf120 ELISA Kit, Vegf164 ELISA Kit, Vegf188 ELISA Kit, Vpf ELISA Kit, VEGF-A ELISA Kit, VEGF164 ELISA Kit, eVEGF120 ELISA Kit, eVEGF164 ELISA Kit, vegf-a ELISA Kit, vegf ELISA Kit, vascular endothelial growth factor A ELISA Kit, vascular endothelial growth factor A L homeolog ELISA Kit, vascular endothelial growth factor ELISA Kit, vascular endothelial growth factor precursor ELISA Kit, VEGFA ELISA Kit, Vegfa ELISA Kit, vegfa.L ELISA Kit, VEGF ELISA Kit, vegf ELISA Kit

Gen-ID: 22339

NCBI Accession: NP_001020421

UniProt: Q00731