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CCL22 ELISA Kit

Maus CCL22 ELISA-Kit Colorimetric-Assay zur Quantifizierung von Maus CCL22.
Rockland
Produktnummer ABIN6719956
Hersteller Produkt- Nr.: koa0256

Kurzübersicht für CCL22 ELISA Kit (ABIN6719956)

Target

Alle CCL22 ELISA Kits anzeigen
CCL22 (Chemokine (C-C Motif) Ligand 22 (CCL22))

Reaktivität

  • 6
  • 4
  • 3
  • 2
  • 2
  • 2
  • 2
  • 2
  • 2
  • 1
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  • 1
Maus

Nachweismethode

Colorimetric

Methodentyp

Sandwich ELISA

Detektionsbereich

15.6 pg/mL - 1000 pg/mL

Applikation

ELISA

Proben

Cell Culture Supernatant, Plasma (EDTA - heparin), Serum
  • Untere Nachweisgrenze

    15.6 pg/mL

    Hersteller Produkt- Nr.

    koa0256

    Hersteller

    Rockland

    Verwendungszweck

    Mouse MDC Sandwich ELISA Kit for Quantitative Detection

    Marke

    AccuSignal™

    Analytische Methode

    Quantitative

    Spezifität

    Production: Natural and recombinant mouse MDC. There is no detectable cross-reactivity with other relevant proteins.

    Sensitivität

    < 1 pg/mL

    Bestandteile

    • Antibody-coated 96-well plate
    • Target Protein Standard
    • Detection antibody
    • Detection reagent
    • Diluent buffers
    • Wash buffers
    • Substrate Solution
    • Stop solutions
    • Adhesive covers
  • Applikationshinweise

    Useful in Sandwich ELISA for Quantitative Detection of Antigen. Aliquot 0.1 mL per well of the 1000pg/mL, 500pg/mL, 250pg/mL, 125pg/mL, 62.5pg/mL, 31.2pg/mL, 15.6pg/mL mouse MDC standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of mouse cell culture supernates, serum or plasma(heparin, EDTA) to each empty well. It is recommended that each mouse MDC standard solution and each sample be measured in duplicate.

    Kommentare

    Standard: Expression system for standard: E.coli, Immunogen sequence:  G25-S92

    Probenmenge

    100 μL

    Plattentyp

    Pre-coated

    Beschränkungen

    Nur für Forschungszwecke einsetzbar
  • Lagerung

    4 °C,-20 °C

    Informationen zur Lagerung

    Store vials at 4°C prior to opening. Centrifuge product if not completely clear after standing at room temperature. This product is stable for 6 months at 4°C as an undiluted liquid. Dilute only prior to immediate use. For extended storage freeze at -20°C or below for 12 months. Avoid cycles of freezing and thawing.

    Haltbarkeit

    12 months
  • Target Alle CCL22 ELISA Kits anzeigen

    CCL22 (Chemokine (C-C Motif) Ligand 22 (CCL22))

    Andere Bezeichnung

    MDC

    Hintergrund

    Synonyms: A-152E5.1, ABCD 1, ABCD1, C-C motif chemokine 22, CC chemokine STCP-1, Ccl22, CCL22_HUMAN, Chemokine (C-C motif) ligand 22, DC/B-CK, Macrophage-derived chemokine, MDC(1-69), MDC(7-69), MGC34554, SCYA22, Small inducible cytokine A22, Small inducible cytokine subfamily A (Cys-Cys), member 22, Small-inducible cytokine A22, STCP 1, STCP1, Stimulated T cell chemotactic protein 1, Stimulated T-cell chemotactic protein 1

    Background: Macrophage-derived chemokine(MDC), also called Chemokine, cc motif, ligand 22(CCL22) or Small inducible cytokine subfamily A, member 22(SCY22). MDC is a recently identified member of the CC chemokine family. It is not closely related to other chemokines, sharing most similarity with thymus- and activation-regulated chemokine(TARC), which contains 37 % identical amino acids. In addition, MDC gene is mapped to chromosome 16q13, the same position reported for the TARC gene. MDC has the four-cysteine motif and other highly conserved residues characteristic of CC chemokines, but it shares<35 % identity with any of the known chemokines. Recombinant MDC was expressed in Chinese hamster ovary cells and purified by heparin-Sepharose chromatography. MDC is highly expressed in macrophages and in monocyte-derived dendritic cells, but not in monocytes, natural killer cells, or several cell lines of epithelial, endothelial, or fibroblast origin. High expression was also detected in normal thymus and less expression in lung and spleen. MDC is thus a unique member of the CC chemokine family that may play a fundamental role in the function of dendritic cells, natural killer cells, and monocytes.

    Gen-ID

    20299

    NCBI Accession

    NP_033163

    UniProt

    O88430
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