CXCL9 ELISA Kit

Produktnummer ABIN6719936

Hersteller Produkt- Nr.: koa0428

Dieses Colorimetric ELISA-Kit wurde entwickelt für die quantitative Messung von Maus CXCL9.

Kurzübersicht für CXCL9 ELISA Kit (ABIN6719936)

Target: CXCL9 (gamma-Interferon-Induced Monokine (CXCL9))

Reaktivität: Maus

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Detektionsbereich: 62.5 pg/mL - 4000 pg/mL

Applikation: ELISA

Proben: Cell Culture Supernatant, Plasma (EDTA), Serum

Produktdetails CXCL9 ELISA Kit

Untere Nachweisgrenze: 62.5 pg/mL

Hersteller Produkt- Nr.: koa0428

Hersteller: Rockland

Verwendungszweck: Mouse CXCL9 Sandwich ELISA Kit for Quantitative Detection

Marke: AccuSignal™

Analytische Methode: Quantitative

Spezifität:

Production: Natural and recombinant mouse CXCL9. There is no detectable cross-reactivity with other relevant proteins.

Sensitivität: < 10 pg/mL

Bestandteile:

• Antibody-coated 96-well plate
• Target Protein Standard
• Detection antibody
• Detection reagent
• Diluent buffers
• Wash buffers
• Substrate Solution
• Stop solutions
• Adhesive covers

Anwendungsinformationen

Applikationshinweise:

ELISA_Dilution: 31.2 pg/mL-2000 pg/mL

Application_Note: Useful in Sandwich ELISA for Quantitative Detection of Antigen. Aliquot 0.1 mL per well of the 4000pg/mL, 2000pg/mL,1000pg/mL, 500pg/mL, 250pg/mL, 125pg/mL, and 62.5pg/mL mouse CXCL9 standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of mouse cell culture supernates, serum or plasma(EDTA) to each empty well. It is recommended that each mouse CXCL9 standard solution and each sample be measured in duplicate.

Kommentare:

Standard: Expression system for standard: E.coli, Immunogen sequence: T22-T126

Probenmenge: 100 μL

Plattentyp: Pre-coated

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store vials at 4°C prior to opening. Centrifuge product if not completely clear after standing at room temperature. This product is stable for 6 months at 4°C as an undiluted liquid. Dilute only prior to immediate use. For extended storage freeze at -20°C or below for 12 months. Avoid cycles of freezing and thawing.

Haltbarkeit: 12 months

Details zu CXCL9

Target: CXCL9 (gamma-Interferon-Induced Monokine (CXCL9))

Andere Bezeichnung: CXCL9

Hintergrund:

Synonyms: C-X-C motif chemokine 9, Chemokine (C-X-C motif) ligand 9, CMK, Crg 10, CXCL9, CXCL9_HUMAN, Gamma interferon induced monokine, Gamma-interferon-induced monokine, HuMIG, MIG, Monokine induced by gamma interferon, Monokine induced by interferon gamma, Monokine induced by interferon-gamma, SCYB9, Small inducible cytokine B9, Small inducible cytokine subfamily B member 9, Small-inducible cytokine B9

Background: Chemokine (C-X-C motif) ligand 9 (CXCL9) is a small cytokine belonging to the CXC chemokine family that is also known as Monokine induced by gamma interferon (MIG). CXCL9 is a T-cell chemoattractant, which is induced by IFN-gamma. It is closely related to two other CXC chemokines called CXCL10 and CXCL11, whose genes are located near the gene for CXCL9 on human chromosome 4. CXCL9, CXCL10 and CXCL11 all elicit their chemotactic functions by interacting with the chemokine receptor CXCR3. The standard product used in this kit is recombinant human CXCL9, consisting of 103 amino acids with the molecular mass of 11.7KDa.

Gen-ID: 17329

NCBI Accession: NP_032625

UniProt: P18340