IGF2R ELISA Kit

Produktnummer ABIN6719898

Hersteller Produkt- Nr.: koa0796

Dieses Human IGF2R ELISA-Kit ist ein Colorimetric ELISA-Kit, das dafür entwickelt wurde, Human IGF2R zu quantifizieren.

Kurzübersicht für IGF2R ELISA Kit (ABIN6719898)

Target: IGF2R (Insulin-Like Growth Factor 2 Receptor (IGF2R))

Reaktivität: Human

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Detektionsbereich: 156 pg/mL - 10000 pg/mL

Applikation: ELISA

Proben: Cell Culture Lysate, Cell Culture Supernatant, Plasma (EDTA - heparin), Serum, Tissue Homogenate

Produktdetails IGF2R ELISA Kit

Untere Nachweisgrenze: 156 pg/mL

Hersteller Produkt- Nr.: koa0796

Hersteller: Rockland

Verwendungszweck: Human IGF2R Sandwich ELISA Kit for Quantitative Detection

Marke: AccuSignal™

Analytische Methode: Quantitative

Spezifität:

Production: Natural and recombinant human IGF2R. There is no detectable cross-reactivity with other relevant proteins.

Sensitivität: < 10 pg/mL

Bestandteile:

• Antibody-coated 96-well plate
• Target Protein Standard
• Detection antibody
• Detection reagent
• Diluent buffers
• Wash buffers
• Substrate Solution
• Stop solutions
• Adhesive covers

Anwendungsinformationen

Applikationshinweise: Useful in Sandwich ELISA for Quantitative Detection of Antigen. Aliquot 0.1 mL per well of the 10,000pg/mL, 5000pg/mL, 2500pg/mL, 1250pg/mL, 625pg/mL, 312pg/mL, 156pg/mL human IGF2R standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of human cell culture supernates, serum or plasma(heparin, EDTA) to each empty well. It is recommended that each human IGF2R standard solution and each sample be measured in duplicate.

Kommentare:

Standard: Expression system for standard: NSO, Immunogen sequence: S1510-F2108

Probenmenge: 100 μL

Plattentyp: Pre-coated

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store vials at 4°C prior to opening. Centrifuge product if not completely clear after standing at room temperature. This product is stable for 6 months at 4°C as an undiluted liquid. Dilute only prior to immediate use. For extended storage freeze at -20°C or below for 12 months. Avoid cycles of freezing and thawing.

Haltbarkeit: 12 months

Details zu IGF2R

Target: IGF2R (Insulin-Like Growth Factor 2 Receptor (IGF2R))

Andere Bezeichnung: IGF2R

Hintergrund:

Synonyms: 300 kDa mannose 6 phosphate receptor, Cation independent mannose 6 phosphate receptor, CD222, CD222 antigen, CI Man 6 P receptor, CI-M6PR, CI-MPR, CIMPR, IGF 2 receptor, IGF 2R, IGF II receptor, IGF2 receptor, IGF2R, Insulin like growth factor 2 receptor, Insulin like growth factor II receptor, M6P R, M6P/IGF2 receptor, M6P/IGF2R, M6PR, mannose 6 phosphate receptor, mannose 6 phosphate receptor cation independent, MPR300, MPRI, MPRI_HUMAN

Background: Insulin-like growth factor 2 receptor, also called IGF2R or I-MPR is a protein that in humans is encoded by the IGF2R gene. This gene is mapped to 6q25.3. This gene encodes a receptor for both insulin-like growth factor 2 and mannose 6-phosphate, although the binding sites for either are located on different segments of the receptor. This receptor functions in the intracellular trafficking of lysosomal enzymes, the activation of transforming growth factor beta, and the degradation of insulin-like growth factor 2. While the related mouse gene shows exclusive expression from the maternal allele, imprinting of the human gene appears to be polymorphic, with only a minority of individuals showing expression from the maternal allele.

Gen-ID: 3482

NCBI Accession: NP_000867

UniProt: P11717