Galectin 2 ELISA Kit

Produktnummer ABIN6719894

Hersteller Produkt- Nr.: koa0754

Dieses Colorimetric ELISA-Kit wurde entwickelt für die quantitative Messung von Human Galectin 2.

Kurzübersicht für Galectin 2 ELISA Kit (ABIN6719894)

Target: Galectin 2 (LGALS2) (Lectin, Galactoside-Binding, Soluble, 2 (LGALS2))

Reaktivität: Human

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Detektionsbereich: 156 pg/mL - 10000 pg/mL

Applikation: ELISA

Proben: Cell Culture Lysate, Cell Culture Supernatant, Plasma (EDTA - heparin), Serum, Tissue Homogenate

Produktdetails Galectin 2 ELISA Kit

Untere Nachweisgrenze: 156 pg/mL

Hersteller Produkt- Nr.: koa0754

Hersteller: Rockland

Verwendungszweck: Human Galectin-2 Sandwich ELISA Kit for Quantitative Detection

Marke: AccuSignal™

Analytische Methode: Quantitative

Spezifität:

Production: Natural and recombinant human Galectin-2. There is no detectable cross-reactivity with other relevant proteins.

Sensitivität: < 10 pg/mL

Bestandteile:

• Antibody-coated 96-well plate
• Target Protein Standard
• Detection antibody
• Detection reagent
• Diluent buffers
• Wash buffers
• Substrate Solution
• Stop solutions
• Adhesive covers

Anwendungsinformationen

Applikationshinweise:

ELISA_Dilution: 156pg/mL-10,000pg/mL

Application_Note: Useful in Sandwich ELISA for Quantitative Detection of Antigen. Aliquot 0.1 mL per well of the 10,000pg/mL, 5000pg/mL, 2500pg/mL, 1250pg/mL, 625pg/mL, 312pg/mL, 156pg/mL human Galectin-2 standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of human cell culture supernates, serum or plasma(heparin, EDTA) to each empty well. It is recommended that each human Galectin-2 standard solution and each sample be measured in duplicate.

Kommentare:

Standard: Expression system for standard: E.coli, Immunogen sequence: M1-E132

Probenmenge: 100 μL

Plattentyp: Pre-coated

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store vials at 4°C prior to opening. Centrifuge product if not completely clear after standing at room temperature. This product is stable for 6 months at 4°C as an undiluted liquid. Dilute only prior to immediate use. For extended storage freeze at -20°C or below for 12 months. Avoid cycles of freezing and thawing.

Haltbarkeit: 12 months

Details zu Galectin 2

Target: Galectin 2 (LGALS2) (Lectin, Galactoside-Binding, Soluble, 2 (LGALS2))

Andere Bezeichnung: Galectin-2

Hintergrund:

Synonyms: Beta galactoside binding lectin L14 II, Beta-galactoside-binding lectin L-14-II, CBP35, Gal-2, GAL3, GALBP, Galectin 2, Galectin-2, GALIG, HL14, Lactose binding lectin 2, Lactose-binding lectin 2, LEG2_HUMAN, Lgals2, MAC2, S Lac lectin 2, S-Lac lectin 2

Background: Galectin-2 is a protein that in humans is encoded by the LGALS2 gene. It is mapped to 22q13.1. The protein encoded by this gene is a soluble beta-galactoside binding lectin. The encoded protein is found as a homodimer and can bind to lymphotoxin-alpha. It has been found that a single-nucleotide polymorphism in Galectin-2 is significantly associated with susceptibility to myocardial infarction. This genetic substitution affects the transcriptional level of Galectin-2 in vitro, potentially leading to altered secretion of LTA, to which Galectin-2 binds, which would then affect the degree of inflammation.

Gen-ID: 3957

NCBI Accession: NP_006489

UniProt: P05162