MADCAM1 ELISA Kit

Produktnummer ABIN6719835

Hersteller Produkt- Nr.: koa0801

Dieses Maus MADCAM1 ELISA-Kit ist ein Colorimetric ELISA-Kit, das dafür entwickelt wurde, Maus MADCAM1 zu quantifizieren.

Kurzübersicht für MADCAM1 ELISA Kit (ABIN6719835)

Target: MADCAM1 (Mucosal Vascular Addressin Cell Adhesion Molecule 1 (MADCAM1))

Reaktivität: Maus

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Detektionsbereich: 125 pg/mL - 8000 pg/mL

Applikation: ELISA

Proben: Cell Culture Supernatant, Serum

Produktdetails MADCAM1 ELISA Kit

Untere Nachweisgrenze: 125 pg/mL

Hersteller Produkt- Nr.: koa0801

Hersteller: Rockland

Verwendungszweck: Mouse MADCAM-1 Sandwich ELISA Kit for Quantitative Detection

Marke: AccuSignal™

Analytische Methode: Quantitative

Spezifität:

Production: Natural and recombinant mouse MADCAM-1. There is no detectable cross-reactivity with other relevant proteins.

Sensitivität: < 10 pg/mL

Bestandteile:

• Antibody-coated 96-well plate
• Target Protein Standard
• Detection antibody
• Detection reagent
• Diluent buffers
• Wash buffers
• Substrate Solution
• Stop solutions
• Adhesive covers

Anwendungsinformationen

Applikationshinweise: Useful in Sandwich ELISA for Quantitative Detection of Antigen. Aliquot 0.1 mL per well of the 8000pg/mL, 4000pg/mL, 2000pg/mL, 1000pg/mL, 500pg/mL, 250pg/mL, 125pg/mL mouse MADCAM-1 standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of mouse cell culture supernates or serum to each empty well. It is recommended that each mouse MADCAM-1 standard solution and each sample be measured in duplicate.

Kommentare:

Standard: Expression system for standard: NSO, Immunogen sequence: Q22-T365

Probenmenge: 100 μL

Plattentyp: Pre-coated

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store vials at 4°C prior to opening. Centrifuge product if not completely clear after standing at room temperature. This product is stable for 6 months at 4°C as an undiluted liquid. Dilute only prior to immediate use. For extended storage freeze at -20°C or below for 12 months. Avoid cycles of freezing and thawing.

Haltbarkeit: 12 months

Details zu MADCAM1

Target: MADCAM1 (Mucosal Vascular Addressin Cell Adhesion Molecule 1 (MADCAM1))

Andere Bezeichnung: MADCAM-1

Hintergrund:

Synonyms: Addressin mucosal, hMAdCAM 1, hMAdCAM-1, MACAM1, MADCA_HUMAN, MAdCAM 1, MAdCAM-1, Madcam1, Mucosal addressin cell adhesion molecule 1, Mucosal vascular addressin cell adhesion molecule 1

Background: MADCAM1(Mucosal Vascular Addressin Cell Adhesion Molecule 1), also known as MACAM1, is a protein that is encoded by the MADCAM1 gene. By PCR-based analysis of somatic cell hybrids, Leung et al(1997) mapped the MACAM1 gene to chromosome 19. The protein encoded by this gene is an endothelil cell adhesion molecule that interacts preferentially with the leukocyte beta7 integrin LPAM-1(alpha4 / beta7), L-selectin, and VLA-4(alpha4/beta1) on myeloid cells to direct leukocytes into mucosal and inflamed tissues. It is a member of the immunoglobulin superfamily and is similar to ICAM-1 and VCAM-1.

Gen-ID: 17123

NCBI Accession: NP_038619

UniProt: Q61826