CD163 ELISA Kit

Produktnummer ABIN6719823

Hersteller Produkt- Nr.: koa0417

Dieses Human CD163 ELISA-Kit ist ein Colorimetric ELISA-Kit, das dafür entwickelt wurde, Human CD163 zu quantifizieren.

Kurzübersicht für CD163 ELISA Kit (ABIN6719823)

Target: CD163

Reaktivität: Human

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Detektionsbereich: 1.56 ng/mL - 100 ng/mL

Applikation: ELISA

Proben: Cell Culture Supernatant, Plasma (heparin), Serum

Produktdetails CD163 ELISA Kit

Untere Nachweisgrenze: 1.56 ng/mL

Hersteller Produkt- Nr.: koa0417

Hersteller: Rockland

Verwendungszweck: Human CD163 Sandwich ELISA Kit for Quantitative Detection

Marke: AccuSignal™

Analytische Methode: Quantitative

Spezifität:

Production: Natural and recombinant human CD163. There is no detectable cross-reactivity with other relevant proteins.

Sensitivität: < 150 pg/mL

Bestandteile:

• Antibody-coated 96-well plate
• Target Protein Standard
• Detection antibody
• Detection reagent
• Diluent buffers
• Wash buffers
• Substrate Solution
• Stop solutions
• Adhesive covers

Anwendungsinformationen

Applikationshinweise: Useful in Sandwich ELISA for Quantitative Detection of Antigen. Aliquot 0.1 mL per well of the 100 ng/mL,50 ng/mL, 25 ng/mL,12.5 ng/mL,6.25 ng/mL,3.13 ng/mL,1.56 ng/mL human CD163 standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of human cell culture supernatants, serum or plasma(heparin) to each empty well. We recommend that each human CD163 standard solution and each sample is measured in duplicate.

Kommentare:

Standard: Expression system for standard: NSO, Immunogen sequence: G41-S1045

Probenmenge: 100 μL

Plattentyp: Pre-coated

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store vials at 4°C prior to opening. Centrifuge product if not completely clear after standing at room temperature. This product is stable for 6 months at 4°C as an undiluted liquid. Dilute only prior to immediate use. For extended storage freeze at -20°C or below for 12 months. Avoid cycles of freezing and thawing.

Haltbarkeit: 12 months

Details zu CD163

Target: CD163

Andere Bezeichnung: CD163

Hintergrund:

Synonyms: CD_antigen=CD163, CD163, Hemoglobin scavenger receptor, M130, MM130, Scavenger receptor cysteine-rich type 1 protein M130

Background: CD163(Cluster of Differentiation 163) is a human protein encoded by the CD163 gene. It has also been shown to mark cells of monocyte/macrophage lineage. CD163, a member of the scavenger receptor cysteine-rich(SRCR) superfamily, is exclusively expressed by monocytes and macrophages. Using FISH, somatic cell hybrid analysis, and radiation hybrid analysis, CD163 gene was mapped the to chromosome 12p13.3. CD163 is upregulated in a large range of diseases inflammatory diseases including type 2 diabetes, macrophage activation sickness, Tangier's disease, rheumatoid arthritis etc.

Gen-ID: 9332

NCBI Accession: NP_004235

UniProt: Q86VB7