RAGE ELISA Kit

Produktnummer ABIN6719822

Produktdetails RAGE ELISA Kit

Target: RAGE (AGER) (Advanced Glycosylation End Product-Specific Receptor (AGER))

Reaktivität: Human

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Detektionsbereich: 78 pg/mL - 5000 pg/mL

Untere Nachweisgrenze: 78 pg/mL

Applikation: ELISA

Verwendungszweck: Sandwich ELISA for Quantitative Detection of Antigen

Proben: Cell Culture Supernatant, Plasma (EDTA - heparin), Serum

Analytische Methode: Quantitative

Produktmerkmale:

Synonyms: Advanced glycosylation end product specific receptor, Advanced glycosylation end product-specific receptor, Ager, DAMA 358M23.4, LE 9211 A antigen, MAPK MAK MRK overlapping kinase, MGC22357, MOK, OTTHUMP00000174885, RAGE 1, RAGE isoform NtRAGE-delta, RAGE isoform sRAGE-delta, RAGE_HUMAN, RAGE1, Receptor for advanced glycation end products, receptor for advanced glycation end-products variant 20, Receptor for advanced glycosylation end products, Renal tumor antigen, Renal tumor antigen 1

Background: RAGE, the Receptor for Advanced Glycation Endproducts, is a 35kD transmembrane receptor of the immunoglobulin super family. It is also known as ""AGER"". AGER gene is mapped to chromosome 6p21.3 by mapping by contiguous cosmids and YAC clones and by fluorescence in situ hybridization. The expression of RAGE is particularly increased in neurons close to deposits of amyloid beta peptide and to neurofibrillary tangles. RAGE has been linked to several chronic diseases, which are thought to result from vascular damage. The pathogenesis is hypothesized to include ligand binding upon which RAGE signals activation of the nuclear factor kappa B(NF-kappaB).

Gene Name: AGER

Production: Natural and recombinant human Rage. There is no detectable cross-reactivity with other relevant proteins.

Standard: Expression system for standard: NSO, Immunogen sequence: Q24-A344

Anwendungsinformationen

Applikationshinweise:

Application Note: Useful in Sandwich ELISA for Quantitative Detection of Antigen. Aliquot 0.1 mL per well of the 5000pg/mL, 2500pg/mL, 1250pg/mL, 625pg/mL, 312pg/mL, 156pg/mL, 78pg/mL human Rage standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of human cell culture supernatants, serum or plasma(heparin, EDTA) to each empty well. We recommend that each human Rage standard solution and each sample is measured in duplicate.

Blood Product Anticoagulant: Heparin Sodium

ELISA Dilution: 78pg/mL-5000pg/mL

Probenmenge: 100 μL

Plattentyp: Pre-coated

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Lagerung: RT,4 °C,-20 °C

Informationen zur Lagerung: Store vials at 4°C prior to opening. Centrifuge product if not completely clear after standing at room temperature. This product is stable for 6 months at 4°C as an undiluted liquid. Dilute only prior to immediate use. For extended storage freeze at -20°C or below for 12 months. Avoid cycles of freezing and thawing.

Details zu RAGE

Target: RAGE (AGER) (Advanced Glycosylation End Product-Specific Receptor (AGER))

Andere Bezeichnung: Rage (AGER Produkte)

Synonyme: RAGE ELISA Kit, AGER ELISA Kit, advanced glycosylation end-product specific receptor ELISA Kit, advanced glycosylation end product-specific receptor ELISA Kit, MAPK/MAK/MRK overlapping kinase ELISA Kit, AGER ELISA Kit, Ager ELISA Kit, LOC719012 ELISA Kit

Gen-ID: 177

NCBI Accession: NP_001127

UniProt: B4DNX3

Pathways: Carbohydrate Homeostasis, Toll-Like Receptors Cascades, Smooth Muscle Cell Migration, S100 Proteine