BMP7 ELISA Kit

Produktnummer ABIN6719808

Hersteller Produkt- Nr.: koa0533

Dieses Human BMP7 ELISA-Kit ist ein Colorimetric ELISA-Kit, das dafür entwickelt wurde, Human BMP7 zu quantifizieren.

Kurzübersicht für BMP7 ELISA Kit (ABIN6719808)

Target: BMP7 (Bone Morphogenetic Protein 7 (BMP7))

Reaktivität: Human

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Detektionsbereich: 31.2 pg/mL - 2000 pg/mL

Applikation: ELISA

Proben: Bone, Cell Culture Supernatant, Plasma (EDTA - heparin), Serum

Produktdetails BMP7 ELISA Kit

Untere Nachweisgrenze: 31.2 pg/mL

Hersteller Produkt- Nr.: koa0533

Hersteller: Rockland

Verwendungszweck: Human BMP-7 Sandwich ELISA Kit for Quantitative Detection

Marke: AccuSignal™

Analytische Methode: Quantitative

Spezifität:

Production: Natural and recombinant human BMP-7. There is no detectable cross-reactivity with other relevant proteins.

Sensitivität: < 10 pg/mL

Bestandteile:

• Antibody-coated 96-well plate
• Target Protein Standard
• Detection antibody
• Detection reagent
• Diluent buffers
• Wash buffers
• Substrate Solution
• Stop solutions
• Adhesive covers

Anwendungsinformationen

Applikationshinweise: Useful in Sandwich ELISA for Quantitative Detection of Antigen. Aliquot 0.1 mL per well of the 2000pg/mL,1000pg/mL, 500pg/mL, 250pg/mL, 125pg/mL, 62.5pg/mL, 31.2pg/mL human BMP-7 standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of human bone tissue, cell culture supernates, serum, plasma((heparin, EDTA) or urine to each empty well. It is recommended that each human BMP-7 standard solution and each sample be measured in duplicate.

Kommentare:

Standard: Expression system for standard: NSO, Immunogen sequence: S293-H431

Probenmenge: 100 μL

Plattentyp: Pre-coated

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store vials at 4°C prior to opening. Centrifuge product if not completely clear after standing at room temperature. This product is stable for 6 months at 4°C as an undiluted liquid. Dilute only prior to immediate use. For extended storage freeze at -20°C or below for 12 months. Avoid cycles of freezing and thawing.

Haltbarkeit: 12 months

Details zu BMP7

Target: BMP7 (Bone Morphogenetic Protein 7 (BMP7))

Andere Bezeichnung: BMP-7

Hintergrund:

Synonyms: BMP 7, Bone morphogenetic protein 7, Eptotermin alfa, OP 1, OP1, Osteogenic protein 1, OTTHUMP00000166094

Background: Bone morphogenetic protein 7 or BMP7(also known as osteogenic protein-1 or OP-1) is a protein that in humans is encoded by the BMP7 gene. The protein encoded by this gene is a member of the TGF-beta superfamily. Like other members of the bone morphogenetic protein family of proteins, it plays a key role in the transformation of mesenchymal cells into bone and cartilage. It is inhibited by noggin and a similar protein, chordin, which are expressed in the Spemann-Mangold Organizer. BMP7 may be involved in bone homeostasis. It is expressed in the brain, kidneys and bladder.

Gen-ID: 655

NCBI Accession: NP_001710

UniProt: A8K571

Pathways: Steroid Hormone Mediated Signaling Pathway, Stem Cell Maintenance