TNC ELISA Kit

Produktnummer ABIN6719799

Hersteller Produkt- Nr.: koa0673

Dieses Human TNC ELISA-Kit ist ein Colorimetric ELISA-Kit, das dafür entwickelt wurde, Human TNC zu quantifizieren.

Kurzübersicht für TNC ELISA Kit (ABIN6719799)

Target: TNC (Tenascin C (TNC))

Reaktivität: Human

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Detektionsbereich: 93.7 pg/mL - 6000 pg/mL

Applikation: ELISA

Proben: Cell Culture Supernatant, Plasma (EDTA - heparin), Serum, Urine

Produktdetails TNC ELISA Kit

Untere Nachweisgrenze: 93.7 pg/mL

Hersteller Produkt- Nr.: koa0673

Hersteller: Rockland

Verwendungszweck: Human Tenascin-C - TNC Sandwich ELISA Kit for Quantitative Detection

Marke: AccuSignal™

Analytische Methode: Quantitative

Spezifität:

Production: Natural and recombinant human Tenascin-C. There is no detectable cross-reactivity with other relevant proteins.

Sensitivität: < 10 pg/mL

Bestandteile:

• Antibody-coated 96-well plate
• Target Protein Standard
• Detection antibody
• Detection reagent
• Diluent buffers
• Wash buffers
• Substrate Solution
• Stop solutions
• Adhesive covers

Anwendungsinformationen

Applikationshinweise: Useful in Sandwich ELISA for Quantitative Detection of Antigen. Aliquot 0.1 mL per well of the 6000pg/mL, 3000pg/mL, 1500pg/mL, 750pg/mL, 419pg/mL, 187.5pg/mL, 93.7pg/m human Tenascin-C standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of human cell culture supernates, serum, plasma(heparin, EDTA) or urine to each empty well. It is recommended that each human Tenascin-C standard solution and each sample be measured in duplicate.

Kommentare:

Standard: Expression system for standard: NSO, Immunogen sequence: G23-P625

Probenmenge: 100 μL

Plattentyp: Pre-coated

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store vials at 4°C prior to opening. Centrifuge product if not completely clear after standing at room temperature. This product is stable for 6 months at 4°C as an undiluted liquid. Dilute only prior to immediate use. For extended storage freeze at -20°C or below for 12 months. Avoid cycles of freezing and thawing.

Haltbarkeit: 12 months

Details zu TNC

Target: TNC (Tenascin C (TNC))

Andere Bezeichnung: Tenascin-C/TNC

Hintergrund:

Synonyms: 150 225, Cytotactin, Glioma associated extracellular matrix antigen, Glioma-associated-extracellular matrix antigen, GMEM, GP 150 225, GP 150-225 GP, Hexabrachion, HXB, JI, MGC167029, Miotendinous antigen, Myotendinous antigen, Neuronectin, TENA_HUMAN, Tenascin, Tenascin-C, Tenascin-C isoform 14/AD1/16, TenascinC, TN, TN C, TN-C, TNC

Background: Tenascin C (TN-C) is a glycoprotein that in humans is encoded by the TNC gene. It is expressed in the extracellular matrix of various tissues during development, disease or injury, and in restricted neurogenic areas of the central nervous system. Tenascin-C is the founding member of the gene family (Tenascin). In the embryo it is made by migrating cells like the neural crest, it is also abundant in developing tendons, bone and cartilage. TN-C clearly plays a role in cell signaling as evidenced by its ability to be induced during events such as trauma, inflammation, or cancer development. Also, TN-C is important in regulating cell proliferation and migration, especially during developmental differentiation and wound healing.

Gen-ID: 3371

NCBI Accession: NP_002151

UniProt: P24821

Pathways: Regulation of Muscle Cell Differentiation, Regulation of Cell Size, Skeletal Muscle Fiber Development