PLGF ELISA Kit

Produktnummer ABIN6719793

Hersteller Produkt- Nr.: koa0292

Dieses Human PLGF ELISA-Kit ist ein Colorimetric ELISA-Kit, das dafür entwickelt wurde, Human PLGF zu quantifizieren.

Kurzübersicht für PLGF ELISA Kit (ABIN6719793)

Target: PLGF (PGF) (Placenta Growth Factor (PGF))

Reaktivität: Human

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Detektionsbereich: 15.6 pg/mL - 1000 pg/mL

Applikation: ELISA

Proben: Cell Culture Supernatant, Plasma (EDTA - heparin - citrate), Serum, Urine

Produktdetails PLGF ELISA Kit

Untere Nachweisgrenze: 15.6 pg/mL

Hersteller Produkt- Nr.: koa0292

Hersteller: Rockland

Verwendungszweck: Human PLGF Sandwich ELISA Kit for Quantitative Detection

Marke: AccuSignal™

Analytische Methode: Quantitative

Spezifität:

Production: Natural and recombinant human PLGF. There is no detectable cross-reactivity with other relevant proteins.

Sensitivität: < 1 pg/mL

Bestandteile:

• Antibody-coated 96-well plate
• Target Protein Standard
• Detection antibody
• Detection reagent
• Diluent buffers
• Wash buffers
• Substrate Solution
• Stop solutions
• Adhesive covers

Anwendungsinformationen

Applikationshinweise:

ELISA_Dilution: 15.6pg/mL-1000pg/mL

Application_Note: Useful in Sandwich ELISA for Quantitative Detection of Antigen. Aliquot 0.1 mL per well of the 1000pg/mL, 500pg/mL, 250pg/mL, 125pg/mL, 62.5pg/mL, 31.2pg/mL, 15.6pg/mL human PLGF standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of human cell culture supernates, serum, plasma(heparin, EDTA, citrate) or urine to each empty well. It is recommended that each human PLGF standard solution and each sample be measured in duplicate.

Kommentare:

Standard: Expression system for standard: E.coli, Immunogen sequence: A21-R149

Probenmenge: 100 μL

Plattentyp: Pre-coated

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store vials at 4°C prior to opening. Centrifuge product if not completely clear after standing at room temperature. This product is stable for 6 months at 4°C as an undiluted liquid. Dilute only prior to immediate use. For extended storage freeze at -20°C or below for 12 months. Avoid cycles of freezing and thawing.

Haltbarkeit: 12 months

Details zu PLGF

Target: PLGF (PGF) (Placenta Growth Factor (PGF))

Andere Bezeichnung: PLGF

Hintergrund:

Synonyms: AI854365, D12S1900, MGC93298, Pgf, PGFL, PIGF, Placenta growth factor, Placental growth factor, Placental growth factor vascular endothelial growth factor related protein, PlGF, PlGF 2, PLGF_HUMAN, PlGF2, SHGC 10760, Vascular endothelial growth factor related protein

Background: The protein, also called PGF, is 149 amino acids long and shares 53 % identity with the platelet-derived growth factor-like region of human VPF. And the N-glycosylated PLGF protein is secreted into the medium and that it functions as a dimer1. The PLGF gene is mapped to 14q24-q31. There are 3 isoforms of PGF, designated PGF1, PGF2, and PGF3. Only PGF2 is able to bind heparin. Additionally, PGF regulates inter- and intramolecular cross-talk between the VEGF receptor tyrosine kinases FLT1 and FLK12. It also can stimulate angiogenesis and collateral growth in ischemic heart and limb with at least a comparable efficiency to VEGF3.

Gen-ID: 5228

NCBI Accession: NP_001193941

UniProt: P49763

Pathways: VEGFR1 Specific Signals