Soluble Tumor Necrosis Factor Receptor Type 2 (sTNF-R2) ELISA Kit

Produktnummer ABIN6719786

Hersteller Produkt- Nr.: koa0322

Produktdetails

Target: Soluble Tumor Necrosis Factor Receptor Type 2 (sTNF-R2)

Reaktivität: Human

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Detektionsbereich: 7.8 pg/mL - 500 pg/mL

Untere Nachweisgrenze: 7.8 pg/mL

Applikation: ELISA

Hersteller Produkt- Nr.: koa0322

Hersteller: Rockland

Verwendungszweck: Human sTNFsR II Sandwich ELISA Kit for Quantitative Detection

Marke: AccuSignal™

Proben: Cell Culture Supernatant, Plasma (EDTA - heparin - citrate), Serum, Urine

Analytische Methode: Quantitative

Spezifität:

Production: Natural and recombinant human sTNFsRII. There is no detectable cross-reactivity with other relevant proteins.

Sensitivität: < 2 pg/mL

Bestandteile:

• Antibody-coated 96-well plate
• Target Protein Standard
• Detection antibody
• Detection reagent
• Diluent buffers
• Wash buffers
• Substrate Solution
• Stop solutions
• Adhesive covers

Anwendungsinformationen

Applikationshinweise: Useful in Sandwich ELISA for Quantitative Detection of Antigen. Aliquot 0.1 mL per well of the 500pg/mL, 250pg/mL, 125pg/mL, 62.5pg/mL, 31.3pg/mL, 15.6pg/mL, 7.8pg/mL human sTNFsRII standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of human cell culture supernates, serum, plasma( heparin, EDTA, citrate) or urine to each empty well. We recommend that each human sTNFsRII standard solution and each sample is measured in duplicate.

Kommentare:

Standard: Expression system for standard: NSO, Immunogen sequence: L23-D257

Probenmenge: 100 μL

Plattentyp: Pre-coated

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store vials at 4°C prior to opening. Centrifuge product if not completely clear after standing at room temperature. This product is stable for 6 months at 4°C as an undiluted liquid. Dilute only prior to immediate use. For extended storage freeze at -20°C or below for 12 months. Avoid cycles of freezing and thawing.

Haltbarkeit: 12 months

Antigendetails

Target: Soluble Tumor Necrosis Factor Receptor Type 2 (sTNF-R2)

Andere Bezeichnung: sTNFsR II (sTNF-R2 Produkte)

Hintergrund:

Synonyms: CD120b, Etanercept, p75, p80 TNF alpha receptor, TBP2, TBPII, TNF R2, TNFBR, TNFR-II, TNFR2, TNFRII, Tumor necrosis factor binding protein 2, Tumor necrosis factor receptor 2, Tumor necrosis factor receptor superfamily member 1B, Tumor necrosis factor receptor superfamily member 1b, membrane form, Tumor necrosis factor receptor type II

Background: Tumor necrosis factor receptor p75(TNF-R p75, or TNF-R2) is a 75- kDa type I transmembrane protein expressed predominantly on cells of hematopoietic lineage. TNF-R p75 belongs to the TNF receptor superfamily characterized by cysteine-rich extracellular regions composed of three to six disulfide-linked domains. The tumor necrosis factor receptor II(TNFRII) gene localizes to 1p36. 2, a genomic region characteristically deleted in neuroblastomas and other malignancies. In addition, TNFRII is the principal mediator of the effects of TNF on cellular immunity, and it may cooperate with TNFRI in the killing of nonlymphoid cells. The standard product used in this kit is recombinant human sTNFRII, consisting of 24-206 amino acid sequence with the molecular mass of 20KDa.

Gen-ID: 7133

NCBI Accession: NP_001057

UniProt: P20333