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VEGF ELISA Kit

Dieses Colorimetric ELISA-Kit wurde entwickelt für die quantitative Messung von Human VEGF.
Rockland
Produktnummer ABIN6719756
Hersteller Produkt- Nr.: koa0329

Kurzübersicht für VEGF ELISA Kit (ABIN6719756)

Target

Alle VEGF ELISA Kits anzeigen
VEGF (Vascular Endothelial Growth Factor (VEGF))

Reaktivität

  • 6
  • 5
  • 5
  • 2
  • 2
  • 2
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
Human

Nachweismethode

Colorimetric

Methodentyp

Sandwich ELISA

Detektionsbereich

31.2 pg/mL - 2000 pg/mL

Applikation

ELISA

Proben

Cell Culture Supernatant, Plasma (EDTA - heparin - citrate), Serum
  • Untere Nachweisgrenze

    31.2 pg/mL

    Hersteller Produkt- Nr.

    koa0329

    Hersteller

    Rockland

    Verwendungszweck

    Human VEGF Sandwich ELISA Kit for Quantitative Detection

    Marke

    AccuSignal™

    Analytische Methode

    Quantitative

    Spezifität

    Production: Natural and recombinant human VEGF. There is no detectable cross-reactivity with other relevant proteins.

    Sensitivität

    < 1 pg/mL

    Bestandteile

    • Antibody-coated 96-well plate
    • Target Protein Standard
    • Detection antibody
    • Detection reagent
    • Diluent buffers
    • Wash buffers
    • Substrate Solution
    • Stop solutions
    • Adhesive covers
  • Applikationshinweise

    Useful in Sandwich ELISA for Quantitative Detection of Antigen. Aliquot 0.1 mL per well of the 2000pg/mL, 1000pg/mL, 500pg/mL, 250pg/mL, 125pg/mL, 62.5pg/mL, 31.2pg/mL human VEGF standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of human cell culture supernates, serum or plasma(heparin, EDTA, citrate) to each empty well. We recommend that each human VEGF standard solution and each sample is measured in duplicate.

    Kommentare

    Standard: Expression system for standard: sf21, Immunogen sequence: A27-R191

    Detection Range: 31.2 pg/ml - 2000 pg/ml (serum, plasma), 15.6 pg/ml - 1000 pg/ml (cell culture supernates)

    Probenmenge

    100 μL

    Plattentyp

    Pre-coated

    Beschränkungen

    Nur für Forschungszwecke einsetzbar
  • Lagerung

    4 °C,-20 °C

    Informationen zur Lagerung

    Store vials at 4°C prior to opening. Centrifuge product if not completely clear after standing at room temperature. This product is stable for 6 months at 4°C as an undiluted liquid. Dilute only prior to immediate use. For extended storage freeze at -20°C or below for 12 months. Avoid cycles of freezing and thawing.

    Haltbarkeit

    12 months
  • Target Alle VEGF ELISA Kits anzeigen

    VEGF (Vascular Endothelial Growth Factor (VEGF))

    Andere Bezeichnung

    VEGF

    Hintergrund

    Synonyms: MGC70609, MVCD1, Vascular endothelial growth factor, Vascular endothelial growth factor A, Vascular permeability factor, VEGF A, VEGFA, VPF

    Background: Vascular permeability factor/vascular endothelial growth factor(VPF/VEGF), a potent cytokine expressed by most malignant tumors, has critical roles in vasculogenesis and both physiological and pathological angiogenesis. VEGF produced by tumor cells potently stimulates endothelial cell proliferation and angiogenesis and plays a key role in the pathophysiology of several neoplasias. VEGF may also play a pivotal role in mediating the development and progression of diabetic retinopathy. VEGF, a major regulator of angiogenesis, binds to two receptor tyrosine kinases, KDR/Flk-1 and Flt-1. The VEGF gene is mapped by fluorescence in situ hybridization to chromosome 6p12.

    Gen-ID

    7422

    NCBI Accession

    NP_001020537

    UniProt

    P15692
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