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G-CSF ELISA Kit (Colony Stimulating Factor 3 (Granulocyte)) ELISA Kit

CSF3 Reaktivität: Human Colorimetric Sandwich ELISA 31.2 pg/mL - 2000 pg/mL Cell Culture Supernatant, Plasma (EDTA - heparin - citrate), Serum
Produktnummer ABIN6719749
$529.00
Zzgl. Versandkosten $45.00
1 kit
Lieferung in 1 bis 2 Werktagen
  • Target Alle G-CSF (CSF3) ELISA Kits anzeigen
    G-CSF (CSF3)
    Reaktivität
    • 8
    • 5
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Human
    Nachweismethode
    Colorimetric
    Methodentyp
    Sandwich ELISA
    Detektionsbereich
    31.2 pg/mL - 2000 pg/mL
    Untere Nachweisgrenze
    31.2 pg/mL
    Applikation
    ELISA
    Verwendungszweck
    Sandwich ELISA for Quantitative Detection of Antigen
    Proben
    Cell Culture Supernatant, Plasma (EDTA - heparin - citrate), Serum
    Analytische Methode
    Quantitative
    Produktmerkmale

    Synonyms: C17orf33, Colony stimulating factor 3 (granulocyte), CSF 3, CSF beta, CSF3, CSF3OS, Csfg, Filgrastim, GCSA, GCSF, Granulocyte colony stimulating fact, Lenograstim, Macrophage granulocyte inducer 2, MGC45931, MGI 2, MGI IG, OTTMUSP00000006785, Pluripoietin, RP23-395E10.4

    Background: Granulocyte colony-stimulating factor(G-CSF) is a member of the CSF family of hormone-like glycoprotein that regulates hematopoietic cell proliferation and differentiation, and it almost exclusively stimulates the colony formation of granulocytes from committed precursor cells in semi-solid agar culture. G-CSF is also termed colony stimulating factor-3, and a single gene of which codes for a 177 or 180 amino acid mature protein of molecular weight 19,600. Functionally, it specifically stimulates the proliferation and differentiation of the progenitor cells for granulocytes. The effect of G-CSF on myeloid leukemias is unique among colony stimulating factors in driving the leukemic cells from a self-renewing malignant state to a mature differentiated phenotype with the concomitant loss of tumorigenicity. Besides, it also prevents cardiac remodeling after myocardial infarction by activating the Jak-Stat pathway in cardiomyocytes. The recombinant form of hG-CSF is capable of supporting neutrophil proliferation in a CFU-GM assay as well as early erythroid colonies and mixed colony formation. Human gene coding for G-CSF is assigned to the q21-q22 region of chromosome 17. The standard product used in this kit is recombinant human G-CSF, consisting of 175 amino acids with the molecular mass of 18.8KDa.

    Gene Name: CSF3

    Production: Natural and recombinant human G-CSF. There is no detectable cross-reactivity with other relevant proteins.

    Standard: Expression system for standard: E.coli, Immunogen sequence: T31-P204

  • Applikationshinweise

    Application Note: Useful in Sandwich ELISA for Quantitative Detection of Antigen. Aliquot 0.1 mL per well of the 2000pg/mL,1000pg/mL, 500pg/mL, 250pg/mL, 125pg/mL, 62.5pg/mL, 31.2pg/mL human G-CSF standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of human cell culture supernates, serum or plasma(heparin, EDTA, citrate) to each empty well. It is recommended that each human G-CSF standard solution and each sample be measured in duplicate.

    Blood Product Anticoagulant: Heparin Sodium

    ELISA Dilution: 31.2pg/mL-2000pg/mL

    Probenmenge
    100 μL
    Plattentyp
    Pre-coated
    Beschränkungen
    Nur für Forschungszwecke einsetzbar
  • Lagerung
    RT,4 °C,-20 °C
    Informationen zur Lagerung
    Store vials at 4°C prior to opening. Centrifuge product if not completely clear after standing at room temperature. This product is stable for 6 months at 4°C as an undiluted liquid. Dilute only prior to immediate use. For extended storage freeze at -20°C or below for 12 months. Avoid cycles of freezing and thawing.
  • Target Alle G-CSF (CSF3) ELISA Kits anzeigen
    G-CSF (CSF3)
    Andere Bezeichnung
    G-CSF (CSF3 Produkte)
    Synonyme
    CSF3, G-CSF, gcsf, Csfg, MGI-IG, C17orf33, CSF3OS, GCSF, Gcsf, colony stimulating factor 3, colony stimulating factor 3 (granulocyte) a, colony stimulating factor 3 (granulocyte), CSF3, csf3a, Csf3
    Gen-ID
    1440
    NCBI Accession
    NP_000750
    UniProt
    P09919
    Pathways
    Cellular Response to Molecule of Bacterial Origin, Regulation of Actin Filament Polymerization
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