CXCL16 ELISA Kit

Produktnummer ABIN6719735

Hersteller Produkt- Nr.: koa0434

Dieses Colorimetric ELISA-Kit wurde entwickelt für die quantitative Messung von Human CXCL16 in Cell Culture Supernatant, Plasma (EDTA - heparin) und Serum.

Kurzübersicht für CXCL16 ELISA Kit (ABIN6719735)

Target: CXCL16 (Chemokine (C-X-C Motif) Ligand 16 (CXCL16))

Reaktivität: Human

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Detektionsbereich: 93.7 pg/mL - 6000 pg/mL

Applikation: ELISA

Proben: Cell Culture Supernatant, Plasma (EDTA - heparin), Serum

Produktdetails CXCL16 ELISA Kit

Untere Nachweisgrenze: 93.7 pg/mL

Hersteller Produkt- Nr.: koa0434

Hersteller: Rockland

Verwendungszweck: Human CXCL16 Sandwich ELISA Kit for Quantitative Detection

Marke: AccuSignal™

Analytische Methode: Quantitative

Spezifität:

Production: Natural and recombinant human CXCL16. There is no detectable cross-reactivity with other relevant proteins.

Sensitivität: < 10 pg/mL

Bestandteile:

• Antibody-coated 96-well plate
• Target Protein Standard
• Detection antibody
• Detection reagent
• Diluent buffers
• Wash buffers
• Substrate Solution
• Stop solutions
• Adhesive covers

Anwendungsinformationen

Applikationshinweise:

ELISA_Dilution: 93.7pg/mL-6000pg/mL

Application_Note: Useful in Sandwich ELISA for Quantitative Detection of Antigen. Aliquot 0.1 mL per well of the 6000pg/mL, 3000pg/mL, 1500pg/mL, 750pg/mL, 419pg/mL, 187.5pg/mL, 93.7pg/m human CXCL16 standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of human cell culture supernates, serum or plasma(heparin, EDTA) to each empty well. It is recommended that each human CXCL16 standard solution and each sample be measured in duplicate.

Kommentare:

Standard: Expression system for standard: E.coli, Immunogen sequence: N49-P137

Probenmenge: 100 μL

Plattentyp: Pre-coated

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store vials at 4°C prior to opening. Centrifuge product if not completely clear after standing at room temperature. This product is stable for 6 months at 4°C as an undiluted liquid. Dilute only prior to immediate use. For extended storage freeze at -20°C or below for 12 months. Avoid cycles of freezing and thawing.

Haltbarkeit: 12 months

Details zu CXCL16

Target: CXCL16 (Chemokine (C-X-C Motif) Ligand 16 (CXCL16))

Andere Bezeichnung: CXCL16

Hintergrund:

Synonyms: 0910001K24Rik, AV290116, BB024863, C X C motif chemokine 16, Chemokine (C X C motif) ligand 16, CXC chemokine ligand 16, CXCLG16, DN 491F10.6, Scavenger receptor for phosphatidylserine and oxidized low density lipoprotein, SCYB16, Small inducible cytokine B, Small inducible cytokine B16, Small inducible cytokine B16 precursor, SR PSOX, SR PSOX/CXCL16 scavenger receptor for phosphatidylserine and oxidized low density lipoprotein, SRPSOX, Transmembrane chemokine CXCL16, Zinc finger, MYND-type containing 15, Zmynd15

Background: Chemokine(C-X-C motif) ligand 16(CXCL16) is a small cytokine belonging to the CXC chemokine family. Larger than other chemokines(with 254 amino acids), CXCL16 is composed of a CXC chemokine domain, a mucin-like stalk, a transmembrane domain and a cytoplasmic tail containing a potential tyrosine phosphorylation site that may bind SH2. These are unusual features for a chemokine, and allow CXCL16 to be expressed as a cell surface bound molecule, as well as a soluble chemokine. CXCL16 is produced by dendritic cells found in the T cell zones of lymphoid organs, and by cells found in the red pulp of the spleen. Cells that bind and migrate in response to CXCL16 include several subsets of T cells, and natural killer T(NKT) cells. CXCL16 interacts with the chemokine receptorCXCR6, also known as Bonzo. The novel CXC-chemokine ligand 16(CXCL16) functions as transmembrane adhesion molecule on the surface of APCs and as a soluble chemoattractant for activated T cells. Expression of CXCL16 is induced by the inflammatory cytokines IFN-gamma and TNF-alpha. The gene for human CXCL16 is located on chromosome 17.

Gen-ID: 58191

NCBI Accession: NP_001094282

UniProt: Q9H2A7