CCL9 ELISA Kit

Produktnummer ABIN6719734

Produktdetails CCL9 ELISA Kit

Target: CCL9 (Ccl9) (Chemokine (C-C Motif) Ligand 9 (Ccl9))

Reaktivität: Maus

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Detektionsbereich: 7.8 pg/mL - 500 pg/mL

Untere Nachweisgrenze: 7.8 pg/mL

Applikation: ELISA

Verwendungszweck: Sandwich ELISA for Quantitative Detection of Antigen

Proben: Cell Culture Supernatant, Plasma (EDTA - heparin), Serum

Analytische Methode: Quantitative

Produktmerkmale:

Synonyms: CCF18, CCL9, Chemokine CC motif ligand 9, Macrophage Inflammatory Protein 1 gamma, Macrophage inflammatory protein-related protein 2, MIP-1 gamma, mMIP1g, SCYA10, SCYA9, Small inducible cytokine A9

Background: Chemokine (C-C motif) ligand 9(CCL9), also called MIP-1 gamma and MRP-2, is a small cytokine belonging to the CC chemokine family. CCL9 which found around Peyer's patches is secreted by follicle-associated epithelium(FAE), and it can attract dendritic cells that possess the cell surface molecule CD11b and the chemokine receptor CCR1. CCL9 is constitutively expressed in macrophages and myeloid cells. CCL9 can activate osteoclasts through its receptor CCR1(the most abundant chemokine receptor found on osteoclasts) suggesting an important role for CCL9 in bone resorption.

Gene Name: Ccl9

Production: Natural and recombinant mouse CCL9 . There is no detectable cross-reactivity with other relevant proteins.

Standard: Expression system for standard: E.coli, Immunogen sequence: Q22-Q122

Anwendungsinformationen

Applikationshinweise:

Application Note: Useful in Sandwich ELISA for Quantitative Detection of Antigen. Aliquot 0.1 mL per well of the 500pg/mL, 250pg/mL, 125pg/mL, 62.5pg/mL, 31.3pg/mL, 15.6pg/mL, 7.8pg/mL mouse CCL9 standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of mouse cell culture supernates, serum or plasma(heparin, EDTA) to each empty well. We recommend that each mouse CCL9 standard solution and each sample is measured in duplicate.

Blood Product Anticoagulant: Heparin Sodium

ELISA Dilution: 7.8pg/mL-500pg/mL

Probenmenge: 100 μL

Plattentyp: Pre-coated

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Lagerung: RT,4 °C,-20 °C

Informationen zur Lagerung: Store vials at 4°C prior to opening. Centrifuge product if not completely clear after standing at room temperature. This product is stable for 6 months at 4°C as an undiluted liquid. Dilute only prior to immediate use. For extended storage freeze at -20°C or below for 12 months. Avoid cycles of freezing and thawing.

Details zu CCL9

Target: CCL9 (Ccl9) (Chemokine (C-C Motif) Ligand 9 (Ccl9))

Andere Bezeichnung: CCL9 (Ccl9 Produkte)

Synonyme: CCF18 ELISA Kit, MRP-2 ELISA Kit, Scya10 ELISA Kit, Scya9 ELISA Kit, chemokine (C-C motif) ligand 9 ELISA Kit, Ccl9 ELISA Kit

Gen-ID: 20308

NCBI Accession: NP_035468

UniProt: P51670