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PON1 ELISA Kit

Dieses Colorimetric ELISA-Kit wurde entwickelt für die quantitative Messung von Human PON1.
Rockland
Produktnummer ABIN6719699
Hersteller Produkt- Nr.: koa0653

Kurzübersicht für PON1 ELISA Kit (ABIN6719699)

Target

Alle PON1 ELISA Kits anzeigen
PON1 (Paraoxonase 1 (PON1))

Reaktivität

  • 5
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Human

Nachweismethode

Colorimetric

Methodentyp

Sandwich ELISA

Detektionsbereich

31.2 pg/mL - 2000 pg/mL

Applikation

ELISA

Proben

Cell Culture Lysate, Cell Culture Supernatant, Plasma (heparin), Serum, Tissue Homogenate
  • Untere Nachweisgrenze

    31.2 pg/mL

    Hersteller Produkt- Nr.

    koa0653

    Hersteller

    Rockland

    Verwendungszweck

    Human PON1 Sandwich ELISA Kit for Quantitative Detection

    Marke

    AccuSignal™

    Analytische Methode

    Quantitative

    Spezifität

    Production: Natural and recombinant human PON1. There is no detectable cross-reactivity with other relevant proteins.

    Sensitivität

    < 10 pg/mL

    Bestandteile

    • Antibody-coated 96-well plate
    • Target Protein Standard
    • Detection antibody
    • Detection reagent
    • Diluent buffers
    • Wash buffers
    • Substrate Solution
    • Stop solutions
    • Adhesive covers
  • Applikationshinweise

    Useful in Sandwich ELISA for Quantitative Detection of Antigen. Aliquot 0.1 mL per well of the 2000pg/mL,1000pg/mL, 500pg/mL, 250pg/mL, 125pg/mL, 62.5pg/mL, 31.2pg/mL human PON1 standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of human cell culture supernates, cell lysates, serum, plasma(heparin) or tissue homogenates to each empty well. It is recommended that each human PON1 standard solution and each sample be measured in duplicate.

    Kommentare

    Standard: Expression system for standard: NSO, Immunogen sequence: A2-L355

    Probenmenge

    100 μL

    Plattentyp

    Pre-coated

    Beschränkungen

    Nur für Forschungszwecke einsetzbar
  • Lagerung

    4 °C,-20 °C

    Informationen zur Lagerung

    Store vials at 4°C prior to opening. Centrifuge product if not completely clear after standing at room temperature. This product is stable for 6 months at 4°C as an undiluted liquid. Dilute only prior to immediate use. For extended storage freeze at -20°C or below for 12 months. Avoid cycles of freezing and thawing.

    Haltbarkeit

    12 months
  • Target Alle PON1 ELISA Kits anzeigen

    PON1 (Paraoxonase 1 (PON1))

    Andere Bezeichnung

    PON1

    Hintergrund

    Synonyms: A esterase 1, A-esterase 1, Aromatic esterase 1, Arylesterase 1, Arylesterase B type, ESA, Esterase A, K 45, K-45, MVCD5, Paraoxonase 1, Paraoxonase, Paraoxonase B type, Paraoxonase, plasma, Paraoxonase1, PON 1, PON, PON1, PON1_HUMAN, Serum aryldiakylphosphatase, Serum aryldialkylphosphatase 1, Serum paraoxonase/arylesterase 1

    Background: Serum paraoxonase/arylesterase 1(PON1) also known as aromatic esterase 1 or serum aryldialkylphosphatase 1 is an enzyme that in humans is encoded by the PON1 gene. The PON gene was mapped to chromosome 7q21-q22, and the mouse Pon1 gene was mapped to the proximal end of chromosome 6. PON1 is responsible for hydrolysing organophosphate pesticides and nerve gasses. Polymorphisms in the PON1 gene significantly affect the catalytic ability of the enzyme. PON1(paraoxonase 1) is also a major anti-atherosclerotic component of high-density lipoprotein(HDL).

    Gen-ID

    5444

    NCBI Accession

    NP_000437

    UniProt

    P27169
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