SPARCL1 ELISA Kit

Produktnummer ABIN6719689

Hersteller Produkt- Nr.: koa0783

Human SPARCL1 ELISA Kit, Colorimetric Assay für die Quantifizierung von Human SPARCL1.

Kurzübersicht für SPARCL1 ELISA Kit (ABIN6719689)

Target: SPARCL1 (SPARC-Like 1 (Hevin) (SPARCL1))

Reaktivität: Human

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Detektionsbereich: 46.9 pg/mL - 3000 pg/mL

Applikation: ELISA

Proben: Cell Culture Supernatant, Serum

Produktdetails SPARCL1 ELISA Kit

Untere Nachweisgrenze: 46.9 pg/mL

Hersteller Produkt- Nr.: koa0783

Hersteller: Rockland

Verwendungszweck: Human SPARCL1 Sandwich ELISA Kit for Quantitative Detection

Marke: AccuSignal™

Analytische Methode: Quantitative

Spezifität: Expression system for standard: NS0
Immunogen sequence: I17-F664

Kreuzreaktivität (Details): There is no detectable cross-reactivity with other relevant proteins.

Sensitivität: < 10 pg/mL

Bestandteile:

• Antibody-coated 96-well plate
• Target Protein Standard
• Detection antibody
• Detection reagent
• Diluent buffers
• Wash buffers
• Substrate Solution
• Stop solutions
• Adhesive covers

Benötigtes Material: Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional). Pipettes and pipette tips capable of precisely dispensing 0.5 μL through 1 mL volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500 mL graduated cylinders. Test tubes for dilution.

Anwendungsinformationen

Kommentare:

Capture antibody and Detection antibody: monoclonal antibody from mouse, polyclonal antibody from goat

Probenmenge: 100 μL

Plattentyp: Pre-coated

Protokoll: Useful in Sandwich ELISA for Quantitative Detection of Antigen. Aliquot 0.1 mL per well of the 3000pg/mL, 1500pg/mL, 750pg/mL, 419pg/mL, 188pg/mL, 94pg/mL, 46.9pg/mL human SPARCL1 standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of human cell culture supernates or serum to each empty well. We recommend that each human SPARCL1 standard solution and each sample is measured in duplicate. 2. Seal the plate with the cover and incubate at 37 °C for 90 min.

Testpräzision: Intra-Assay Precision (CV%): 5.5%, 5.5%, 6.7%
Inter-Assay Precision (CV%): 6.1%, 6.7%, 7.1%

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store vials at 4°C prior to opening. Centrifuge product if not completely clear after standing at room temperature. This product is stable for 6 months at 4°C as an undiluted liquid. Dilute only prior to immediate use. For extended storage freeze at -20°C or below for 12 months. Avoid cycles of freezing and thawing.

Haltbarkeit: 12 months

Details zu SPARCL1

Target: SPARCL1 (SPARC-Like 1 (Hevin) (SPARCL1))

Andere Bezeichnung: SPARCL1

Hintergrund:

Background: SPARCL1 (SPARC-Like Protein 1), also known as HEVIN, is a protein that in humans is encoded by the SPARCL1 gene. The cells in high endothelial venules (HEVs) in lymphoid tissues have a plump, almost cuboidal, appearance and support high levels of lymphocyte extravasation from blood, possibly due to the presence of desmosome-like junctions rather than tight junctions in the HEVs. In chronic inflammation, the activated endothelium of nonlymphoid tissues acquires an HEV-like morphology and function. SPARCL1 is highly expressed in HEV and is thought to contribute to the induction or maintenance of features of the HEV endothelium that facilitate lymphocyte migration.

Gene Full Name: SPARC like 1

Gen-ID: 8404

NCBI Accession: NP_001121782

UniProt: Q14515