TIMP4 ELISA Kit

Produktnummer ABIN6719680

Produktdetails TIMP4 ELISA Kit

Target: TIMP4 (TIMP Metallopeptidase Inhibitor 4 (TIMP4))

Reaktivität: Maus

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Detektionsbereich: 78 pg/mL - 5000 pg/mL

Untere Nachweisgrenze: 78 pg/mL

Applikation: ELISA

Verwendungszweck: Sandwich ELISA for Quantitative Detection of Antigen

Proben: Cell Culture Supernatant, Plasma (EDTA - heparin), Serum

Analytische Methode: Quantitative

Produktmerkmale:

Synonyms: Metalloproteinase inhibitor 4, TIMP 4, TIMP metallopeptidase inhibitor 4, Tissue inhibitor of metalloproteinase 4, Tissue inhibitor of metalloproteinases 4

Background: Metalloproteinase inhibitor 4 is an enzyme that in humans is encoded by the TIMP4 gene. This gene belongs to the tissue inhibitor of metalloproteinases gene family. The proteins encoded by this gene family are inhibitors of the matrix metalloproteinases, a group of peptidases involved in degradation of the extracellular matrix. TIMP4 gene is mapped to chromosome 3p25. TIMP4 gene contains 5 exons that span 6 kb of genomic DNA. The gene is expressed as a 1.4-kb transcript abundant in heart and present at low levels in several other tissues. Expressed TIMP4 inhibits MMPs in vitro. What's more, TIMP-4 binds both progelatinase A and the C domain in a similar manner to that of TIMP-2.

Gene Name: TIMP4

Production: Natural and recombinant mouse TIMP-4. There is no detectable cross-reactivity with other relevant proteins.

Standard: Expression system for standard: NSO, Immunogen sequence: C30-P224

Anwendungsinformationen

Applikationshinweise:

Application Note: Useful in Sandwich ELISA for Quantitative Detection of Antigen. Aliquot 0.1 mL per well of the 5,000pg/mL, 2500pg/mL, 1250pg/mL, 625pg/mL, 312pg/mL, 156pg/mL, 78pg/mL mouse TIMP-4 standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of mouse cell culture supernatants, serum or plasma(heparin, EDTA) to each empty well. We recommend that each mouse TIMP-4 standard solution and each sample is measured in duplicate.

Blood Product Anticoagulant: Heparin Sodium

ELISA Dilution: 78pg/mL-5,000pg/mL

Probenmenge: 100 μL

Plattentyp: Pre-coated

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Lagerung: RT,4 °C,-20 °C

Informationen zur Lagerung: Store vials at 4°C prior to opening. Centrifuge product if not completely clear after standing at room temperature. This product is stable for 6 months at 4°C as an undiluted liquid. Dilute only prior to immediate use. For extended storage freeze at -20°C or below for 12 months. Avoid cycles of freezing and thawing.

Details zu TIMP4

Target: TIMP4 (TIMP Metallopeptidase Inhibitor 4 (TIMP4))

Andere Bezeichnung: TIMP-4 (TIMP4 Produkte)

Synonyme: TIMP4 ELISA Kit, TIMP-4 ELISA Kit, timp4 ELISA Kit, TIMP metallopeptidase inhibitor 4 ELISA Kit, TIMP metallopeptidase inhibitor 4, tandem duplicate 3 ELISA Kit, tissue inhibitor of metalloproteinase 4 ELISA Kit, TIMP4 ELISA Kit, timp4.3 ELISA Kit, Timp4 ELISA Kit

Gen-ID: 110595

NCBI Accession: NP_542370

UniProt: Q9JHB3