Thyroxine T4 ELISA Kit

Produktnummer ABIN629485

Hormone Thyroxine T4 ELISA-Kit Colorimetric-Assay zur Quantifizierung von Hormone Thyroxine T4.

Kurzübersicht für Thyroxine T4 ELISA Kit (ABIN629485)

Target: Thyroxine T4 (T4)

Reaktivität: Hormone

Nachweismethode: Colorimetric

Methodentyp: Competition ELISA

Applikation: ELISA

Produktdetails Thyroxine T4 ELISA Kit

Analytische Methode: Quantitative

Produktmerkmale: ELISA kit for detection of Thyroxine in the research laboratory
Alternative Names: Free T4 ELISA Kit

Anwendungsinformationen

Applikationshinweise: Optimal conditions to be determined by end user

Plattentyp: Pre-coated

Testdurchführung:

The CBI fT4 is a solid phase competitive ELISA. The samples, assay buffer and T4 enzyme conjugate are added to the wells coated with anti-T4 monoclonal antibody. fT4 in the patient's serum competes with a T4 enzyme (HRP) conjugate for binding sites. Unbound fT4 and T4 enzyme conjugate is washed off by washing buffer. Upon the addition of the substrate, the intensity of color is inversely proportional to the concentration of fT4 in the samples. A standard curve is prepared relating color intensity to the concentration of fT4.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Vorsichtsmaßnahmen: All reagents of this test kit derived from human or animal serum or plasma have been tested and confirmed negative for HIV, and HbsAg. However, there is no test method that can offer complete assurance therefore all reagents, should be treated as potential biohazards in use and for disposal.

Lagerung: 4 °C

Details zu Thyroxine T4

Target: Thyroxine T4 (T4)

Andere Bezeichnung: Thyroxine

Substanzklasse: Amino Acid

Hintergrund: The thyroid hormone, thyroxine (T4) is produced by the thyroid gland. An important component in the synthesis is iodine. The major form of thyroid hormone in the blood is thyroxine (T4). Thyroxine is converted to the active T3 (three to four times more potent than T4) within cells by deiodinases (5'- iodinase). Thyroxine-binding globulin (TGB) is the major carrier protein for circulating thyroid hormone. Only a verysmall fraction of the circulating hormone is free (unbound) - T4 0.03 %.