GATA3 ELISA Kit (GATA Binding Protein 3)
Details for Product GATA3 ELISA Kit No. ABIN627064- Antigen
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Synonyme für dieses Antigen anzeigen
- HDR
- HDRS
- Gata-3
- jal
- cb550
- gta3
- GATA-3
- XGATA3
- xgata-3
- hdr
- xGATA-3
- GATA3
- GATA transcription factor 3
- T4L20.260
- T4L20_260
- gata3
- GATA binding protein 3
- GATA binding protein 3 S homeolog
- GATA transcription factor 3
- amidase
- putative amidase
- GATA-binding protein 3
- GATA3
- Gata3
- gata3
- gata3.S
- gatA3
- Reaktivität
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Ratte (Rattus)
Alternativen - Nachweismethode
- Colorimetric
- Methodentyp
- Sandwich ELISA
- Detektionsbereich
- 1.0-25 ng/mL
- Untere Nachweisgrenze
- 1.0 ng/mL
- Applikation
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ELISA
- Optionen
Applikations-hinweise |
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Kommentare |
Information on standard material: Different kits have different standards. For kits detecting proteisn or peptidse, the standards are recombinant proteins or synthetic peptides. For kits detecting small chemical compounds, the standards are synthetic chemical compounds. There are no standards extracted from natural resources. All of our reombinant proteins are expressed in E.coli. The standard are dissolved in PBS with 0.1 % proclin 300 and some other preservatives. Information on reagents: The STOP solution is 1M sulphuric acid. The wash buffer is 0.05 % Tween 20 in PBS, pH 7.4. The ELISA kit dose not contain (sodium) azide, thimerosal, 2-mercaptoethanol (2-ME). Part of the reagents contain BSA. Information on antibodies: The provided antibodies and their host vary in different kits. |
Probenmenge | 50 μL |
Testdauer | 1.5 h |
Plattentyp | Pre-coated |
Aufbereitung der Reagenzien |
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Probennahme |
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Testdurchführung |
Prepare all Standards before starting assay procedure (Please read Reagents Preparation). It is recommended that all Standards and Samples be added in duplicate to the Microtiter Plate. 1. Secure the desired number of coated wells in the holder then add 50 µL of Standards or Samples to the appropriate well of the antibody pre-coated Microtiter Plate. 2. Add 100 µL of Conjugate to each well. Mix well. Complete mixing in this step is important. Cover and incubate for 1 hour at 37 °C. 3. Wash the Microtiter Plate using one of the specified methods indicated below: 4. Manual Washing: Remove incubation mixture by aspirating contents of the plate into a sink or proper waste container. Using a squirt bottle, fill each well completely with wash solution, then aspirate contents of the plate into a sink or proper waste container. Repeat this procedure four more times for a total of FIVE washes. After final wash, invert plate, and blot dry by hitting plate onto absorbent paper or paper towels until no moisture appears. Note: Hold the sides of the plate frame firmly hen washing the plate to assure that all strips remain securely in frame. 5. Automated Washing: Aspirate all wells, and then wash plate FIVE times using wash solution. Always adjust your washer to aspirate as much liquid as possible and set fill volume at 350 µL/well/wash (range: 350-400 µL). After final wash, invert plate, and blot dry by hitting plate onto absorbent paper or paper towels until no moisture appears. It is recommended that the washer be set for a soaking time of 10 seconds or shaking time of 5 seconds between washes. 6. Add 50 µL Substrate A to each well. 7.Add 50 µL Substrate B to each well. Cover and incubate for 15 minutes at 20-25 °C. (avoid sunlight) 8. Add 50 µL of Stop Solution to each well. Mix well. 9. Read the Optical Density (O.D.) at 450 nm using a microtiter plate reader immediately. |
Ergebnisberechnung |
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Beschränkungen | Nur für Forschungszwecke einsetzbar |
Konservierungs-mittel | Sodium azide |
Vorsichtsmaßnahmen |
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Handhabung |
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Lagerung | 4 °C |
Haltbarkeit | 6 months |
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