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NrCAM ELISA Kit

Dieses Colorimetric ELISA-Kit wurde entwickelt für die quantitative Messung von Human NrCAM in Cell Culture Supernatant, Plasma, Serum.
Produktnummer ABIN625342

Kurzübersicht für NrCAM ELISA Kit (ABIN625342)

Target

Alle NrCAM (NRCAM) ELISA Kits anzeigen
NrCAM (NRCAM) (Neuronal Cell Adhesion Molecule (NRCAM))

Reaktivität

  • 3
  • 1
  • 1
Human

Nachweismethode

Colorimetric

Methodentyp

Sandwich ELISA

Detektionsbereich

0.3-80 ng/mL

Applikation

ELISA

Proben

Cell Culture Supernatant, Plasma, Serum
  • Untere Nachweisgrenze

    0.3 ng/mL

    Verwendungszweck

    Human NrCAM ELISA Kit for cell culture supernatants, plasma, and serum samples.

    Analytische Methode

    Quantitative

    Spezifität

    This ELISA antibody pair detects human NrCAM. Other species not determined.

    Sensitivität

    0.3 ng/mL

    Produktmerkmale

    • Strip plates and additional reagents allow for use in multiple experiments
    • Quantitative protein detection
    • Establishes normal range
    • The best products for confirmation of antibody array data

    Bestandteile

    • Pre-Coated 96-well Strip Microplate
    • Wash Buffer
    • Stop Solution
    • Assay Diluent(s)
    • Lyophilized Standard
    • Biotinylated Detection Antibody
    • Streptavidin-Conjugated HRP
    • TMB One-Step Substrate

    Benötigtes Material

    • Distilled or deionized water
    • Precision pipettes to deliver 2 μL to 1 μL volumes
    • Adjustable 1-25 μL pipettes for reagent preparation
    • 100 μL and 1 liter graduated cylinders
    • Tubes to prepare standard and sample dilutions
    • Absorbent paper
    • Microplate reader capable of measuring absorbance at 450nm
    • Log-log graph paper or computer and software for ELISA data analysis
  • Applikationshinweise

    Recommended Dilution for serum and plasma samples2 fold

    Probenmenge

    100 μL

    Plattentyp

    Pre-coated

    Protokoll

    1. Prepare all reagents, samples and standards as instructed in the manual.
    2. Add 100 μL of standard or sample to each well.
    3. Incubate 2.5 h at RT or O/N at 4 °C.
    4. Add 100 μL of prepared biotin antibody to each well.
    5. Incubate 1 h at RT.
    6. Add 100 μL of prepared Streptavidin solution to each well.
    7. Incubate 45 min at RT.
    8. Add 100 μL of TMB One-Step Substrate Reagent to each well.
    9. Incubate 30 min at RT.
    10. Add 50 μL of Stop Solution to each well.
    11. Read at 450 nm immediately.

    Beschränkungen

    Nur für Forschungszwecke einsetzbar
  • Lagerung

    -20 °C

    Informationen zur Lagerung

    The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.

    Haltbarkeit

    6 months
  • Target Alle NrCAM (NRCAM) ELISA Kits anzeigen

    NrCAM (NRCAM) (Neuronal Cell Adhesion Molecule (NRCAM))

    Andere Bezeichnung

    NrCAM

    Hintergrund

    Gene Names: NRCAM KIAA0343
    Protein names: Neuronal cell adhesion molecule (Nr-CAM) (Neuronal surface protein Bravo) (hBravo) (NgCAM-related cell adhesion molecule) (Ng-CAM-related)

    Gen-ID

    4897

    UniProt

    Q92823

    Pathways

    Regulation of Cell Size
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