XCL1 ELISA Kit

Produktnummer ABIN625333

Produktdetails XCL1 ELISA Kit

Target: XCL1 (Chemokine (C Motif) Ligand 1 (XCL1))

Reaktivität: Human

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Detektionsbereich: 150 pg/mL-50 ng/mL

Untere Nachweisgrenze: 150 pg/mL

Applikation: ELISA

Verwendungszweck: Human Lymphotactin (XCL1) ELISA Kit for cell culture supernatants and serum samples. EDTA is recommended as an anticoagulant for plasma in this array.

Proben: Plasma, Cell Culture Supernatant, Serum

Analytische Methode: Quantitative

Spezifität: This ELISA kit shows no cross-reactivity with the following cytokines tested: human Angiogenin, BDNF, BLC, CNTF, ENA- 78, FGF-4, IL-1 alpha, IL-1 beta, IL-2, IL-3, IL-4, IL-5, IL-6, IL-7, IL-8, IL-9, IL-11, IL-12 p70, IL-12 p40, IL-13, IL-15, I-309, IP-10, FGF-4, FGF-6, FGF- 7, G-CSF, GDNF, GM-CSF, IFN-gamma, IGFBP-2, IGFBP-3, IGFBP-4, Leptin (OB), MCP-1, MCP-2, MCP-3, MDC, MIF, MIG, MIP-1 alpha, MIP-1 beta, MIP-1 delta, PARC, PDGF, RANTES, SCF, SDF-1 alpha, TARC, TGF-beta, TIMP-1, TIMP-2, TNF-alpha, TNF-beta, TPO, VEGF.

Sensitivität: 150 pg/mL

Produktmerkmale:

• Strip plates and additional reagents allow for use in multiple experiments
• Quantitative protein detection
• Establishes normal range
• The best products for confirmation of antibody array data

Bestandteile:

• Pre-Coated 96-well Strip Microplate
• Wash Buffer
• Stop Solution
• Assay Diluent(s)
• Lyophilized Standard
• Biotinylated Detection Antibody
• Streptavidin-Conjugated HRP
• TMB One-Step Substrate

Benötigtes Material:

• Distilled or deionized water
• Precision pipettes to deliver 2 μL to 1 μL volumes
• Adjustable 1-25 μL pipettes for reagent preparation
• 100 μL and 1 liter graduated cylinders
• Tubes to prepare standard and sample dilutions
• Absorbent paper
• Microplate reader capable of measuring absorbance at 450nm
• Log-log graph paper or computer and software for ELISA data analysis

Anwendungsinformationen

Applikationshinweise: Recommended Dilution for serum and plasma samples2 fold

Probenmenge: 100 μL

Plattentyp: Pre-coated

Protokoll:

1. Prepare all reagents, samples and standards as instructed in the manual.
2. Add 100 μL of standard or sample to each well.
3. Incubate 2.5 h at RT or O/N at 4 °C.
4. Add 100 μL of prepared biotin antibody to each well.
5. Incubate 1 h at RT.
6. Add 100 μL of prepared Streptavidin solution to each well.
7. Incubate 45 min at RT.
8. Add 100 μL of TMB One-Step Substrate Reagent to each well.
9. Incubate 30 min at RT.
10. Add 50 μL of Stop Solution to each well.
11. Read at 450 nm immediately.

Aufbereitung der Proben:

EDTA is recommended as an anticoagulant for plasma in this array.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Lagerung: -20 °C

Informationen zur Lagerung: The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.

Haltbarkeit: 6 months

Details zu XCL1

Target: XCL1 (Chemokine (C Motif) Ligand 1 (XCL1))

Andere Bezeichnung: Lymphotactin (XCL1 Produkte)

Synonyme: ATAC ELISA Kit, LPTN ELISA Kit, LTN ELISA Kit, SCM-1 ELISA Kit, SCM-1a ELISA Kit, SCM1 ELISA Kit, SCM1A ELISA Kit, SCYC1 ELISA Kit, AI661682 ELISA Kit, Lptn ELISA Kit, Scyc1 ELISA Kit, Ltn ELISA Kit, XCL1 ELISA Kit, XCL2 ELISA Kit, X-C motif chemokine ligand 1 ELISA Kit, chemokine (C motif) ligand 1 ELISA Kit, cytokine SCM-1 beta ELISA Kit, lymphotactin ELISA Kit, XCL1 ELISA Kit, Xcl1 ELISA Kit, LOC490356 ELISA Kit, LOC100714756 ELISA Kit, LOC100349255 ELISA Kit

Hintergrund: Gene Names: XCL1 LTN SCYC1
Protein names: Lymphotactin (ATAC) (C motif chemokine 1) (Cytokine SCM-1) (Lymphotaxin) (SCM-1-alpha) (Small-inducible cytokine C1) (XC chemokine ligand 1)

Gen-ID: 6375

UniProt: P47992

Pathways: Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, Activated T Cell Proliferation